Glia-dependent neurotoxicity and neuroprotection in mesencephalic cultures
Glia-dependent neurotoxicity and neuroprotection in mesencephalic cultures
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DOI：
10.1016/0006-8993(95)01189-7
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发表时间：
1995-12-15
期刊：
影响因子：
2.9
通讯作者：
McMillian, MK
中科院分区：
文献类型：
--
作者：
Bronstein, DM;PerezOtano, I;McMillian, MK
比较6-羟基多巴胺(6-OHDA)和脂多糖对培养的大鼠中脑细胞在多聚L赖氨酸和胶质细胞单层上的多巴胺能神经毒性作用。在聚赖氨酸上的神经元培养中，6-OHDA杀死了89%的酪氨酸羟基酶(TH)免疫阳性神经元，但内毒素没有神经毒性。相反，在神经元/神经胶质混合培养中，6-OHDA仅杀死27%的TH免疫阳性神经元，而内毒素杀死70%。混合神经元/胶质细胞中脑培养为研究帕金森病的可能机制提供了一个更好的体外模型。
Dopaminergic neurotoxicities of 6-hydroxydopamine (6-OHDA) and the lipopolysaccharide (LPS) were compared in rat mesencephalic cultures plated on poly-L-lysine or on glial monolayers. In the neuron-enriched cultures plated on polylysine, 6-OHDA killed 89% Of the tyrosine hydroxylase (TH)-immunopositive neurons, but LPS was not neurotoxic. Conversely, in mixed neuron/glial cultures, 6-OHDA killed only 27% of the TH-immunopositive neurons while LPS killed 70%. The mixed neuronal/glial mesencephalic culture offers a better in vitro model for studying possible mechanisms involved in Parkinson's disease.