SPIN90 (SH3 protein interacting with Nck, 90 kDa), an adaptor protein that is developmentally regulated during cardiac myocyte differentiation
SPIN90 (SH3 protein interacting with Nck, 90 kDa), an adaptor protein that is developmentally regulated during cardiac myocyte differentiation
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DOI：
10.1074/jbc.m009411200
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发表时间：
2001-04-20
影响因子：
4.8
通讯作者：
Song, WK
中科院分区：
文献类型：
--
作者：
Lim, CS;Park, ES;Song, WK
在酵母双杂交筛选中，我们以Nck Src同源3 （SH3）结构域为诱饵，从人类心脏文库中分离出cDNA克隆。全长cDNA编码722个氨基酸，鉴定为一个vip54相关基因，包含一个SH3结构域、一个富含脯氨酸的基序、一个富含丝氨酸/苏氨酸的区域和一个长c端疏水区。我们将该蛋白命名为SPIN90 (bar下H3中的(S) (P) bar下蛋白中的(I)与bar下ck中的（N)相互作用，bar下kDa中的（90））。SH3结构域的氨基酸序列与Fyn、Yes和c-Src的氨基酸序列同源性最高。SPIN90在人体组织中广泛表达；特别是，它在心脏、大脑和骨骼肌中高度表达，其表达在心肌细胞分化过程中受到发育调节。在体外，SPIN90能够结合Nck的第一和第三个SH3结构域，并与心肌细胞内的Nck共定位于肌节z盘。此外，针对SPIN90的抗血清治疗破坏了肌节结构，表明该蛋白可能在维持肌节结构和/或肌原纤维组装成肌节中发挥重要作用。
In the yeast two-hybrid screening, we have isolated a cDNA clone from a human heart library using Nck Src homology 3 (SH3) domains as bait. The full-length cDNA, which encoded 722 amino acids, was identified as a VIP54-related gene containing an SH3 domain, proline-rich motifs, a serine/threonine-rich region, and a long C-terminal hydrophobic region. We refer to this protein as SPIN90 ((S) under bar H3 (P) under bar rotein (I) under bar nteracting with (N) under bar ck, (90) under bar kDa), The amino acid sequence of the SH3 domain has the highest homology with those of Fyn, Yes, and c-Src. SPIN90 was broadly expressed in human tissues; in particular, it was highly expressed in heart, brain, and skeletal muscle, and its expression was developmentally regulated during cardiac myocyte differentiation. SPIN90 is able to bind to the first and third SH3 domains of Nck, in vitro, and is colocalized with Nck at sarcomere Z-discs within cardiac myocytes, Moreover, treatment with antisera raised against SPIN90 disrupted sarcomere structure, suggesting that this protein may play an important role in the maintenance of sarcomere structure and/or in the assembly of myofibrils into sarcomeres.