Molecular-engineered polymeric microcapsules assembled from Concanavalin A and glycogen with specific responses to carbohydrates
Molecular-engineered polymeric microcapsules assembled from Concanavalin A and glycogen with specific responses to carbohydrates
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DOI：
10.1039/c1sm05047h
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发表时间：
2011-06
期刊：
影响因子：
3.4
通讯作者：
Yi Zhu;Weijun Tong;Changyou Gao
中科院分区：
文献类型：
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作者：
Yi Zhu;Weijun Tong;Changyou Gao
在生理pH范围内，以植物凝集素Concanavalin A和多糖糖原为原料，根据凝集素-碳水化合物的相互作用，逐层制备出对甘露糖、果糖、葡萄糖及其相应的多糖葡聚糖有特异性反应的中空聚合物微胶囊。多层膜的生长模式受环境pH条件的影响较大。通过在CaCO3微粒上组装和随后的模板去除，获得了具有可变尺寸和壁厚的空心胶囊。制备的胶囊在pH 6-9范围内保持稳定1 h，并能在3 M尿素处理下存活至少1 h。在生理pH条件下，它们对甘露糖、果糖、葡萄糖和葡聚糖（Mw 20 kDa和40 kDa）有特异性反应，甚至被破坏，而对乳糖和半乳糖等其他类型的碳水化合物则没有反应。与环境pH值（pH 6-9）无关，这些反应与碳水化合物的分子量和浓度有关。最后，将该胶囊作为聚苯乙烯-b-丙烯酸（PS-b-PAA）胶束的递送载体，在葡萄糖或葡聚糖（Mw 20 kDa）的触发下部分释放。由于具有良好的稳定性和对各种碳水化合物的特异性反应性，以及装载和释放功能纳米物体的可能性，这些微胶囊有望成为先进药物递送载体和微反应器的候选者。
Hollow polymeric microcapsules with specific responses to mannose, fructose, glucose and its corresponding polysaccharide, dextran, at the physiological pH range were fabricated from Concanavalin A, a plant lectin, and glycogen, a polysaccharide, based on the lectin–carbohydrate interaction in a layer-by-layer fashion. The growth pattern of the multilayer film was largely influenced by the environmental pH condition. Hollow capsules with variable size and wall thickness were obtained by assembly on CaCO3 microparticles and subsequent template removal. The as-prepared capsules remained stable in the pH range 6–9 for 1 h and could survive 3 M urea treatment for at least 1 h. They could specifically respond to or even be destroyed by mannose, fructose, glucose and dextran (Mw 20 kDa and 40 kDa) at the physiological pH condition, but not by other types of carbohydrates such as lactose and galactose. The responses were dependent on both the molecular weight and the concentration of the carbohydrates regardless of the environmental pH condition (pH 6–9). Finally, the capsules were employed as delivery vehicles for poly(styrene-b-acrylic acid) (PS-b-PAA) micelles, which could be partly released under the triggering of glucose or dextran (Mw 20 kDa). With the combination of good stability and specific responsiveness to various carbohydrates, as well as the possibility of loading and releasing functional nano-objects, these microcapsules could be promising candidates as advanced drug delivery carriers and micro-reactors.