Human osteoblasts grow transitional Si/N apatite in quickly osteointegrated Si3N4 cervical insert
Human osteoblasts grow transitional Si/N apatite in quickly osteointegrated Si3N4 cervical insert
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DOI：
10.1016/j.actbio.2017.09.038
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发表时间：
2017-12-01
影响因子：
9.7
通讯作者：
Bal, B. Sonny
中科院分区：
文献类型：
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作者：
Pezzotti, Giuseppe;Oba, Naoki;Bal, B. Sonny
氮化硅（Si3N4）陶瓷具有加速骨修复的表面化学性质，正如先前通过骨肉瘤和间充质细胞的体外实验所证实的那样。硅氮化硅表面释放的硅酸和氮化合物增强了体外细胞活性。这项研究的结果首次证明，先前观察到的骨整合行为在人类环境中具有特殊的化学作用。Si和N元素刺激祖细胞分化和成骨细胞活性，最终导致骨向内生长加速。在分子尺度上，通过结合组织形态学分析、拉曼光谱、傅立叶变换红外光谱和x射线光电子能谱，深入了解了硅离子和氮离子从氮化硅表面释放的影响。对聚醚醚酮（PEEK）脊柱外植体进行的相同分析显示，没有化学变化，而且成骨活性倾向较低。硅和氮是刺激细胞产生具有晶体缺陷的骨磷灰石的关键元素，导致Si3N4生物医学器件的生物活性增强。本研究比较了外植PEEK和Si3N4脊柱垫片的骨整合过程。数据表明，羟基磷灰石在氮化硅生物陶瓷表面的形成是在人体内以一种特殊的机制发生的。硅和氮被纳入骨组织结构，允许非化学计量骨磷灰石的发展和刺激祖细胞分化成骨活性。通过结合组织学分析、拉曼显微光谱、傅里叶变换红外光谱和x射线光电子能谱检测从氮化硅表面释放的硅离子和氮离子。(C) 2017材料学报Elsevier Ltd.出版。版权所有。
Silicon nitride (Si3N4) ceramics possesses surface chemistry that accelerates bone repair, as previously established by in vitro experiments using both osteosarcoma and mesenchymal cells. The release of silicic acid and nitrogen compounds from the surface Si3N4 enhanced in vitro cellular activity. The results of this study demonstrate for the first time that the osseointegration behavior previously observed is operative with a peculiar chemistry within the human milieu. Si and N elements stimulated progenitor cell differentiation and osteoblastic activity, which ultimately resulted in accelerated bone ingrowth. At the molecular scale, insight into the effect of silicon and nitrogen ions released from the Si3N4 surface was obtained through combined histomorphometric analyses, Raman, Fourier-transform-infrared, and X-ray photoelectron spectroscopies. Identical analyses conducted on a polyetheretherketone (PEEK) spinal explant showed no chemical changes and a lower propensity for osteogenic activity. Silicon and nitrogen are key elements in stimulating cells to generate bony apatite with crystallographic imperfections, leading to enhanced bioactivity of Si3N4 biomedical devices.Statement of SignificanceThis research studies osseointegration processes comparing results from explanted PEEK and Si3N4 spinal spacers. Data show that the formation of hydroxyapatite on silicon nitride bio-ceramic surfaces happens with a peculiar mechanism inside the human body. Silicon and nitrogen were incorporated inside the bony tissue structure allowing the developing of off-stoichiometric bony apatite and stimulating progenitor cell differentiationiosteoblastic activity. Silicon and nitrogen ions released from the Si3N4 surface were detected through combined histologic analyses, Raman microspectroscopy, Fourier-transform infrared, and X-ray photoelectron spectroscopies. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.