Use of thin-layer chromatography in the separation of disaccharides resulting from digestion of chondroitin sulphates with chondroitinases.
Use of thin-layer chromatography in the separation of disaccharides resulting from digestion of chondroitin sulphates with chondroitinases.
复制标题
使用薄层色谱法分离用软骨素酶消化硫酸软骨素产生的二糖。
DOI：
10.1016/s0021-9673(00)86291-3
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
D. Allalouf
中科院分区：
文献类型：
--
作者：
L. Wasserman;A. Ber;D. Allalouf
材料与方法硫酸黄黄素4和硫酸黄黄素6购自Miles Labs。(坎,病了。美国)。透明质酸和皮肤硫酸盐由Ciionelli博士提供，芝加哥大学，芝加哥，伊利诺伊州。硫酸软骨素的商业未指定制剂来自nutrition Biochemicals （Cleveland, Ohio， USA; CS-1）和Delta Chemical Works （New York， NY）。美国;CS-2)。软骨素酶ABC、AC和不饱和双糖均来自日本东京Seikagatu Kogyo。预包覆的纤维素F薄层色谱板，0.1 mm厚，由Merck， Darmstadt， GFR提供。所有化学试剂均为分析试剂级。
MATERIALS AND METHODSChondroitin 4-and 6-sulphate were purchased from Miles Labs.(Kankakee, Ill., USA). Hyaluronic acid and dermatan sulphate were kindly supplied by Dr. Ciionelli, University of Chicago, Chicago, Ill., USA Commercial unspecified preparations of chondroitin sulphate were obtained from Nutritional Biochemicals (Cleveland, Ohio, USA; CS-1) and Delta Chemical Works (New York, NY., USA; CS-2). Chondroitinases ABC and AC and unsaturated disaccharides were obtained from Seikagatu Kogyo (Tokyo, Japan). Pre-coated cellulose F TLC pldtes, 0.1 mm thick, were supplied by Merck, Darmstadt, GFR All chemicals were of analytical-reagent grade.