Effects of a Potential Host Gut-Derived Probiotic, Bacillus subtilis 6-3-1, on the Growth, Non-specific Immune Response and Disease Resistance of Hybrid Grouper (Epinephelus fuscoguttatus♀ x Epinephelus lanceolatus♂)
Effects of a Potential Host Gut-Derived Probiotic, Bacillus subtilis 6-3-1, on the Growth, Non-specific Immune Response and Disease Resistance of Hybrid Grouper (Epinephelus fuscoguttatus♀ x Epinephelus lanceolatus♂)
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潜在宿主肠道衍生益生菌枯草芽孢杆菌 6-3-1 对混合石斑鱼（雌性黑点石斑鱼 x 披针石斑鱼雄性）生长、非特异性免疫反应和抗病性的影响
DOI：
10.1007/s12602-021-09768-6
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发表时间：
2021-03-14
影响因子：
4.9
通讯作者：
Zhou, Yongcan
中科院分区：
文献类型：
--
作者：
Liao, Jingqiu;Cai, Yan;Zhou, Yongcan
从768株健康杂交石斑鱼肠道分离菌中成功筛选出一株潜在的宿主源益生菌-枯草芽孢杆菌6-3-1（棕点石斑鱼雌性x披针叶石斑鱼雄性）的多个益生菌特性的体外测定，如非溶血活性、胞外酶活性、对病原体的抑制活性、对胃肠道应激的耐受性、细胞表面疏水性，自身聚集和抗生素敏感性。8周的饲养试验表明，日粮中添加B. subtilis 6-3-1在3个浓度（1 × 10（6）CFU g（-1）（BS 6）; 1 × 10（7）CFU g（-1）（BS 7）; 1 × 10（8）CFU g（-1）（BS 8））下对杂交石斑鱼在不同时间点的生长均有一定的促进作用。第8周末，BS 6和BS 8显著提高了杂交石斑鱼的增重率和特定生长率。与对照组相比，BS 6组和BS 8组消化酶活性均有所提高，但BS 6组淀粉酶活性提高不显著（P > 0.05）。而BS 7的非特异性免疫刺激效果最好。BS 7显著提高血清总蛋白含量、溶菌酶（LZM）、总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和酸性磷酸酶（ACP）水平，BS 6显著提高血清总蛋白含量、LZM活性，BS 8显著提高LZM、呼吸爆发活性。B。subtilis 6-3-1可上调BS 7组小鼠头肾和小肠MyD 88的表达，增加小肠绒毛长度（VL）。上调BS 6组头肾IgM和BS 8组肠道IgM和TLR 1的表达。虽然都是B。枯草芽孢杆菌6-3-1添加组降低了哈维氏弧菌攻击后的累积死亡率，在三个浓度组中，BS 7表现出最好的保护效果。综上所述，BS 7具有增强免疫力、促进肠道健康和抵抗哈维氏弧菌的作用，在杂交石斑鱼养殖中具有很好的应用前景。
A potential host-derived probiotic, Bacillus subtilis 6-3-1, was successfully screened from 768 isolates from the intestines of healthy hybrid grouper (Epinephelus fuscoguttatus female x Epinephelus lanceolatus male) based on multiple probiotic characteristics in vitro assays, such as, non-hemolytic activity, extracellular enzyme activity, inhibitory activity against pathogens, tolerance to gastrointestinal stress, cell surface hydrophobicity, autoaggregation, and antibiotic susceptibility. Eight weeks of feeding trial revealed that dietary supplementation of B. subtilis 6-3-1 at all three concentrations (1 x 10(6) CFU g(-1) as BS6; 1 x 10(7) CFU g(-1) as BS7; 1 x 10(8) CFU g(-1) as BS8) could promote the growth performance of hybrid groupers to a certain extent at different time points. At the end of 8th week, BS6 and BS8 significantly promoted the weight gain rate (WGR), specific growth rate (SGR) of hybrid groupers. The digestive enzyme activities were also increased in BS6 and BS8 groups comparing with those in control group, except that the increase of amylase activities in BS6 was not significant (P > 0.05). However, BS7 showed the best non-specific immunity stimulating effects among the three concentration groups. While BS7 significantly boosted serum total protein contents, lysozyme (LZM), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and acid phosphatase (ACP) levels, BS6 significantly enhanced serum total protein, LZM activity, and BS8 significantly improved LZM, respiratory bursts activity. B. subtilis 6-3-1 up-regulated the expression of MyD88 in head kidney and intestine and increased villi length (VL) in intestine of BS7 group. It also up-regulated the expression of IgM in head kidney in BS6 group and IgM and TLR1 in intestine of BS8 group. Though all B. subtilis 6-3-1 supplemented groups reduced the cumulative mortality rate post-Vibro harveyi-challenge, BS7 showed the best protection effects among the three concentration groups. In conclusion, with its immune promoting, intestine health enhancing, and V. harveyi resisting effects, BS7 show great potential to be used as a probiotic in hybrid grouper culture.