Analysis of serum soluble CD40 ligand (sCD40L) in the patients undergoing allogeneic stem cell transplantation: platelet is a major source of serum sCD40L
Analysis of serum soluble CD40 ligand (sCD40L) in the patients undergoing allogeneic stem cell transplantation: platelet is a major source of serum sCD40L
复制标题
DOI：
10.1111/j.1600-0609.2004.00342.x
复制
发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
Mizutani, S
中科院分区：
文献类型：
--
作者：
Nagasawa, M;Zhu, Y;Mizutani, S
CD40配体(CD40L)不仅表达于活化的T细胞上，也表达于活化的血小板上。在体外，活化的T细胞和血小板通过不明确的蛋白分解过程释放出可溶性CD40配体(SCD40L)。据报道，系统性红斑狼疮、不稳定型心绞痛、原发性血小板增多症和自身免疫性血小板减少性紫癜患者血清中sCD40L水平升高。然而，体内sCD40L的来源尚不清楚。我们检测了异基因干细胞移植患者血清中系列sCD40L，并与活化T细胞的标志物--血小板数和可溶性IL2R进行了比较。SCD40L与血小板数、血小板生成率呈正相关。在严重移植物抗宿主病sIL2R显著升高的病例中，体内sCD40L不升高。这些结果表明，体内的sCD40L主要是从血小板或在血小板产生过程中释放的，而不是从激活的T细胞释放的。
CD40 ligand (CD40L) is expressed not only on activated T cells but also on activated platelets. A soluble CD40 ligand (sCD40L) is released from the activated T cells and platelets by ill-defined proteolytic process in vitro. It has been reported that sCD40L is elevated in the serum of patients with systemic lupus erythematosus, unstable angina, essential thrombocythemia, and autoimmune thrombocytopenic purupura. However, source of sCD40L in vivo remains to be elucidated. We investigated the serial sCD40L in the serum in patients undergoing allogeneic stem cell transplantation and compared with the platelets number and soluble IL2R, which is a marker of activated T cells. The value of sCD40L was well correlated with platelet number or thrombopoiesis. In cases of severe graft vs. host disease with markedly increased sIL2R, sCD40L was not increased in vivo. These results indicate that sCD40L in vivo is released mainly from the platelets or in the process of platelet production but not from the activated T cells.