Endodermal differentiation of human induced pluripotent stem cells using simple dialysis culture system in suspension culture
Endodermal differentiation of human induced pluripotent stem cells using simple dialysis culture system in suspension culture
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DOI：
10.1016/j.reth.2019.05.004
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发表时间：
2019-12-15
影响因子：
4.3
通讯作者：
Sakai, Yasuyuki
中科院分区：
文献类型：
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作者：
Shinohara, Marie;Choi, Hyunjin;Sakai, Yasuyuki
为了制备代谢器官来源的细胞，需要将人诱导的多能干细胞(HiPSCs)分化为确定的内胚层。分化消耗了昂贵的细胞因子和小分子，这阻碍了分化细胞的可制造性。虽然细胞因子和小分子残留或细胞产生自分泌因子，但仍需进行日常的培养液更换，以去除细胞产生的有毒代谢物。在这项研究中，我们开发了一种简单的透析培养系统来提纯在最终内胚层分化过程中的培养液。我们证明了透析培养可以防止细胞损伤，从而去除乳酸。透析培养的HiPSCs，即使在分化后期不补充激活素A，其分化程度也与正常分化相似。在此透析培养体系中，hPSCs可分化为内胚层细胞系，经培养提纯和回收后，可分化为自分泌因子和细胞因子，从而降低分化成本。(C)2019年，日本再生医学学会。爱思唯尔B.V.制作和主办。
A differentiation of human induced pluripotent stem cells (hiPSCs) into definitive endoderm linage is required for a preparation of metabolic organ derived cells. The differentiation consumed high-priced cytokines and small molecules, which have hampered the manufacturability of differentiated cells. Although the cytokines and small molecules are remained or cells produce the autocrine factors, daily culture medium change should be proceeded to remove toxic metabolites generated from cells. In this study, we developed a simple dialysis culture system to refine the medium during definitive endodermal differentiation. We demonstrated that dialysis culture prevented cell damage to remove lactate. The hiPSCs cultured with dialysis also differentiated similarly as usual differentiation without dialysis even if they were not supplied Activin A for latter culture days in the differentiation. With this dialysis culture system, hiPSCs were differentiated into endodermal lineage with medium refinement and recycling and autocrine factors as well as cytokines, which may lead to reduce differentiation cost. (C) 2019, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.