Monoclonal antibody localization of Na+-K+-ATPase in the exocrine pancreas and parotid of the dog.
Monoclonal antibody localization of Na+-K+-ATPase in the exocrine pancreas and parotid of the dog.
复制标题
Na-K-ATP酶在狗的外分泌胰腺和腮腺中的单克隆抗体定位。
DOI：
10.1152/ajpgi.1987.253.2.g99
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Jamieson,JD
中科院分区：
文献类型：
--
作者：
Smith,ZD;Caplan,MJ;Forbush3rd,B;Jamieson,JD
一种对犬Na ~+-K ~+-ATP酶β亚基具有特异性的单克隆抗体已被鉴定，并用于直接定位犬胰腺和腮腺薄冰冻切片中的酶。抗体7- 2 M仅识别钠泵的β亚基，如通过免疫沉淀和免疫印迹所确定的，并且不针对寡糖决定簇。7- 2 M免疫定位于肾小管细胞的相同细胞和亚细胞结构域，如先前表征的针对钠泵α-亚基的其他抗体那样。在胰腺中，发现Na+-K+-ATP酶主要位于中央腺泡和小叶内导管细胞的基底外侧膜上。小叶间导管细胞也表达大量的位于基底外侧的酶，尽管在腺泡细胞上看到相对较少的泵。在腮腺中，在纹状管细胞上观察到大量的Na+-K+-ATP酶，在闰管和浆液性半月板细胞上也观察到高水平。腺泡细胞的Na+-K+-ATP酶水平也较低。在任何情况下，在胰腺或腮腺细胞的顶端膜上都没有发现Na+-K+-ATP酶。这些数据表明，位于导管来源细胞基底外侧质膜上的Na+-K+-ATP酶可能参与胰腺和腮腺的水和电解质分泌。
A monoclonal antibody specific to the beta-subunit of the canine Na+-K+-ATPase has been characterized and used to directly localize the enzyme in thin frozen sections of dog pancreas and parotid. The antibody, 7-2M, recognizes only the beta-subunit of the sodium pump as determined by immunoprecipitation and immunoblot and is not directed against an oligosaccharide determinant. 7-2M immunolocalizes to the same cellular and subcellular domains of renal tubular cells as do other, previously characterized, antibodies directed to the alpha-subunit of the sodium pump. In the pancreas the preponderance of the Na+-K+-ATPase is found on the basolateral membranes of centroacinar and intralobular duct cells. Interlobular duct cells also express a large component of basolaterally located enzyme, although comparatively little pump is seen on acinar cells. In the parotid a large amount of Na+-K+-ATPase is seen on the striated duct cells, with high levels also noted on cells of the intercalated ducts and serous demilunes. Again the acinar cells show comparatively low levels of Na+-K+-ATPase. In no instance is Na+-K+-ATPase found on the apical membranes of pancreas or parotid cells. These data suggest that Na+-K+-ATPase, located on the basolateral plasmalemma of duct-derived cells, may be involved in water and electrolyte secretion from the pancreas and parotid.