Therapeutic effects of bone marrow-derived mesenchymal stem cells on pulmonary impact injury complicated with endotoxemia in rats.
Therapeutic effects of bone marrow-derived mesenchymal stem cells on pulmonary impact injury complicated with endotoxemia in rats.
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DOI：
10.1016/j.intimp.2012.12.008
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发表时间：
2013-02
影响因子：
5.6
通讯作者：
Yu-jin Zhao;Ce Yang;Hai-yan Wang;Hai-sheng Li;J. Du;W. Gu;Jian-xin Jiang
中科院分区：
文献类型：
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作者：
Yu-jin Zhao;Ce Yang;Hai-yan Wang;Hai-sheng Li;J. Du;W. Gu;Jian-xin Jiang
以往的研究表明，急性肺损伤（ALI）与显著的死亡率相关，迄今为止没有有效的药物治疗显示可以逆转自然进展。本研究旨在探讨骨髓间充质干细胞（MSCs）移植是否能恢复ALI患者受损的肺功能和组织结构。采用亚致死性胸部撞击伤+内毒素血症模型，于伤后2 h将MSCs静脉移植于伤后大鼠体内。采集血样进行血气分析。收集支气管肺泡灌洗液（BALF），检测炎性细胞计数和细胞因子（TNF-α、IL-1β、IL-6和IL-10）水平。收集撞击区附近的左肺下叶进行SRY基因分析以及组织学检查和评分。骨髓间充质干细胞植入后，BALF中炎性细胞数量减少。同时，促炎因子TNF-α和IL-6的分泌减少，抗炎因子IL-10的分泌增加。骨髓间充质干细胞移植可改善肺气体交换，减轻肺损伤，降低大鼠死亡率。这些结果表明，基于MSC的措施可能是创伤和内毒素血症诱导的ALI治疗的一个有前途的策略。
Previous studies have demonstrated that acute lung injury (ALI) is associated with significant mortality and so far no effective pharmacotherapy is shown to reverse the natural progression. This study aims to investigate whether the transplantation of bone marrow derived mesenchymal stem cells (MSCs) might restore damaged pulmonary function and tissue structure in ALI. By using a sublethal chest impact injury plus endotoxemia model, MSCs were intravenously transplanted into the injured rats 2h after trauma. The blood samples were obtained for determination of blood gas. Bronchoalveolar lavage fluid (BALF) was collected for analysis of inflammatory cell count and cytokine (TNF-α, IL-1β, IL-6 and IL-10) levels. The left lower lobes of the lungs, nearby the impact zone, were collected for SRY gene analysis and histological examination and scoring. After engraftment of MSCs, the number of inflammatory cells in BALF was decreased. Meanwhile, the secretions of pro-inflammatory TNF-α and IL-6 were alleviated, while the secretion of anti-inflammatory IL-10 was elevated. Engraftment of MSCs improved the pulmonary gas exchanges, alleviated the lung injury, and reduced the rats' mortality. These results suggested that the MSCs based measures might be a promising strategy in trauma and endotoxemia induced ALI treatment.