STIM1-induced precortical and cortical subdomains of the endoplasmic reticulum
STIM1-induced precortical and cortical subdomains of the endoplasmic reticulum
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DOI：
10.1073/pnas.0911280106
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发表时间：
2009-11-17
影响因子：
11.1
通讯作者：
Cosson, Pierre
中科院分区：
文献类型：
--
作者：
Orci, Lelio;Ravazzola, Mariella;Cosson, Pierre
钙库操作的钙进入依赖于一个专门的隔室的形成，该隔室来源于内质网（ER）并与质膜紧密贴壁。在这项研究中，详细的超微结构分析显示存在三种不同的结构来自传统的ER：皮质前ER，皮质ER，薄皮质ER。富含STIM 1的ER的皮质前亚区可以在不接触质膜的情况下形成。在ER钙耗尽时，这些亚结构域移位到质膜以形成皮质ER，其仍然连接到常规ER。薄的皮质ER，耗尽的BiP和剥夺附着的核糖体，可能代表一个专门的区域，致力于钙调节，不从事蛋白质易位和折叠。这些观察结果形成了未来的结构功能分析的基础上皮层ER。
Store-operated calcium entry relies on the formation of a specialized compartment derived from the endoplasmic reticulum (ER) and closely apposed to the plasma membrane. In this study, detailed ultrastructural analysis revealed the existence of three distinct structures derived from conventional ER: precortical ER, cortical ER, and thin cortical ER. Precortical subdomains of the ER enriched in STIM1 can form without contacting the plasma membrane. Upon ER calcium depletion, these subdomains are translocated to the plasma membrane to form cortical ER, which is still connected to the conventional ER. Thin cortical ER, depleted of BiP and deprived of attached ribosomes, may represent a specialized region dedicated to calcium regulation and not engaged in protein translocation and folding. These observations form the basis for future structure-function analysis of cortical ER.