Limiting immunopathology: Interaction between carotenoids and enzymatic antioxidant defences
Limiting immunopathology: Interaction between carotenoids and enzymatic antioxidant defences
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DOI：
10.1016/j.dci.2014.12.007
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发表时间：
2015-04-01
影响因子：
2.9
通讯作者：
Moret, Y.
中科院分区：
文献类型：
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作者：
Babin, A.;Saciat, C.;Moret, Y.
炎症反应过程中活性氧和氮（ROS和RNS）的释放会对宿主组织造成损害，称为免疫病理学，是生态免疫学中的一个重要因素。综合抗氧化系统由内源性抗氧化酶（例如超氧化物歧化酶 SOD 和过氧化氢酶 CAT）和膳食抗氧化剂（例如类胡萝卜素）组成，有助于应对免疫介导的氧化应激。甲壳类动物储存大量膳食类胡萝卜素，原因尚不清楚。虽然作为免疫刺激剂和抗氧化剂，这些色素与抗氧化酶的相互作用仍不清楚。在这里，我们测试了片足类甲壳动物 Gammarus pulex 中添加类胡萝卜素的膳食补充剂与免疫防御之间的相互作用以及抗氧化酶 SOD 和 CAT 的活性。膳食补充剂增加了血淋巴中循环类胡萝卜素和血细胞的浓度，而免疫反应则导致循环类胡萝卜素的消耗和血细胞密度的下降。有趣的是，与对照组相比，补充的伽马射线显示出 SOD 活性下调，但 CAT 活性较高。我们的研究揭示了膳食类胡萝卜素与内源性抗氧化酶的特定相互作用，并进一步强调了类胡萝卜素在甲壳类动物免疫和/或抗氧化机制进化中的潜在重要性。 (C) 2014 Elsevier Ltd. 保留所有权利。
The release of reactive oxygen and nitrogen species (ROS and RNS) during the inflammatory response generates damages to host tissues, referred to as immunopathology, and is an important factor in ecological immunology. The integrated antioxidant system, comprising endogenous antioxidant enzymes (e.g. superoxide dismutase SOD, and catalase CAT) and dietary antioxidants (e.g. carotenoids), helps to cope with immune-mediated oxidative stress. Crustaceans store large amounts of dietary carotenoids for yet unclear reasons. While being immunostimulants and antioxidants, the interaction of these pigments with antioxidant enzymes remains unclear. Here, we tested the interaction between dietary supplementation with carotenoids and immune challenge on immune defences and the activity of the antioxidant enzymes SOD and CAT, in the amphipod crustacean Gammarus pulex. Dietary supplementation increased the concentrations of circulating carotenoids and haemocytes in the haemolymph, while the immune response induced the consumption of circulating carotenoids and a drop of haemocyte density. Interestingly, supplemented gammarids exhibited down-regulated SOD activity but high CAT activity compared to control ones. Our study reveals specific interactions of dietary carotenoids with endogenous antioxidant enzymes, and further underlines the potential importance of carotenoids in the evolution of immunity and/or of antioxidant mechanisms in crustaceans. (C) 2014 Elsevier Ltd. All rights reserved.