Inhibitory effect of phenolic acids on the proliferation of 3T3-L1 preadipocytes in relation to their antioxidant activity
Inhibitory effect of phenolic acids on the proliferation of 3T3-L1 preadipocytes in relation to their antioxidant activity
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DOI：
10.1021/jf0609882
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发表时间：
2006-06-14
影响因子：
6.1
通讯作者：
Yen, Gow-Chin
中科院分区：
文献类型：
--
作者：
Hsu, Chin-Lin;Huang, Shih-Li;Yen, Gow-Chin
肥胖是世界公共卫生和预防医学的一个重要课题。抑制前脂肪细胞增殖在减肥的机制中起着重要的作用。在这项体外研究中，酚酸对3 T3-L1前脂肪细胞的抑制作用进行了评估，然后进行了相关性分析。结果表明，在生长培养基中添加酚酸类物质会降低3 T3-L1前脂肪细胞的细胞群体生长。绿原酸、没食子酸、邻香豆酸和间香豆酸对3 T3-L1前脂肪细胞的IC 50值分别为72.3、43.3、48.2和49.2 μ M。相关分析表明，酚酸类物质对细胞生长的影响与其抗氧化活性之间存在显著的线性相关关系（r=0.77，p < 0.01）。细胞周期分析表明，用绿原酸、邻香豆酸和间香豆酸处理3 T3-L1前脂肪细胞导致细胞周期停滞在G（1）期。没食子酸不影响细胞周期特征;然而，它以时间和剂量依赖性方式增加凋亡细胞（亚G（1）期）的数量。Annexin V-异硫氰酸荧光素（FITC）、碘化丙啶（PI）凋亡流式细胞仪检测显示没食子酸可增加早期凋亡细胞（annexin-FITC+/ PI-）和晚期凋亡细胞（annexinV-FITC +/ PI+）的数量，但不增加坏死细胞（annexinV-FITC-/PI+）的数量。用没食子酸处理细胞引起线粒体膜电位（Delta Psi（m））的损失。这些结果表明，抑制前脂肪细胞的人口增长的一些酚酸可能有进一步的含义在体内减肥效果。
Obesity is an important topic in the world of public health and preventive medicine. Inhibition of preadipocyte proliferation plays an important role in the mechanisms of proposed antiobesity. In this in vitro study, the inhibitory effect of phenolic acids on 3T3-L1 preadipocytes was evaluated, and a relationship analysis was then conducted. The results showed that the addition of phenolic acids to the growth medium decreased the cell population growth of 3T3-L1 preadipocytes. The IC50 values of chlorogenic acid, gallic acid, o- coumaric acid and m-coumaric acid on 3T3-L1 preadipocytes were 72.3, 43.3, 48.2, and 49.2 mu M, respectively. A relationship analysis indicated that there is a significant linear correlation between the influence of phenolic acids on cell population growth and their antioxidant activity (r=0.77, p < 0.01). The cell cycle assay indicated that the treatment of 3T3-L1 preadipocytes with chlorogenic acid, o- coumaric acid, and m-coumaric acid caused cell cycle arrest in the G(1) phase. Gallic acid did not affect the cell cycle profile; however, it increased the number of apoptotic cells (sub-G(1) phase) in a time- and dose- dependent manner. Annexin V- fluorescein isothiocyanate (FITC) propidium iodide (PI) apoptosis flow cytometric assay showed that gallic acid increased the number of early apoptotic (annexin-FITC+/ PI-) and late apoptotic cells (annexin V-FITC+/ PI+) but not necrotic cells (annexin V- FITC-/PI+). The treatment of cells with gallic acid caused the loss of mitochondrial membrane potential (Delta Psi(m)). These results indicate that the inhibition of preadipocyte population growth by some phenolic acids might have further implication in in vivo antiobesity effects.