Bacteriophages of Erwinia amylovora
Bacteriophages of Erwinia amylovora
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DOI：
10.1128/aem.69.4.2133-2138.2003
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发表时间：
2003-04-01
影响因子：
4.4
通讯作者：
Castle, AJ
中科院分区：
文献类型：
--
作者：
Gill, JJ;Svircev, AM;Castle, AJ
从安大略省南部尼亚加拉地区和安大略省汉密尔顿皇家植物园及其周围的地点收集了50个淀粉欧文氏菌噬菌体分离物，该菌是火灾疫病的病原菌。42个噬菌体在分离、纯化和储存过程中幸存下来。收集的大部分噬菌体是从出现火灾症状的树木周围的土壤中分离出来的。从梨园和苹果园受感染的气生组织中仅分离到5个噬菌体。为了避免任何单一宿主选择的偏见，在最初的分离和浓缩过程中使用了6个细菌宿主菌株。用聚合酶链式反应和限制性内切酶消化相结合的方法对噬菌体进行分子鉴定，结果显示回收了6种不同的噬菌体类型，分别描述为组1到组6。10个噬菌体分离物与已鉴定的淀粉菌PEa1相关，不同地点分离的噬菌体分子标记有一定的差异。对噬菌体寄主范围的研究表明，某些类型的噬菌体不能有效地裂解某些淀粉菌菌株，而某些分离物能够裂解附生细菌聚集性泛胞杆菌。用电子显微镜对六个分子基团的代表进行了研究，以确定它们的形态。在电子显微镜下观察，这些噬菌体显示出不同的形态。组I和组2的噬菌体尾部呈收缩状，组3~组6的噬菌体尾巴较短或开口较细，附属物较薄。根据形态分型，淀粉菌的噬菌体分别隶属于Myoviridae和Podoviridae的尾状病毒目。
Fifty bacteriophage isolates of Erwinia amylovora, the causal agent of fire blight, were collected from sites in and around the Niagara region of southern Ontario and the Royal Botanical Gardens, Hamilton, Ontario. Forty-two phages survived the isolation, purification, and storage processes. The majority of the phages in the collection were isolated from the soil surrounding trees exhibiting fire blight symptoms. Only five phages were isolated from infected aerial tissue in pear and apple orchards. To avoid any single-host selection bias, six bacterial host strains were used in the initial isolation and enrichment processes. Molecular characterization of the phages with a combination of PCR and restriction endonuclease digestions showed that six distinct phage types, described as groups 1 to 6, were recovered. Ten phage isolates were related to the previously characterized E. amylovora PEa1, with some divergence of molecular markers between phages isolated from different sites. A study of the host ranges of the phages revealed that certain types were unable to efficiently lyse some E. amylovora strains and that some isolates were able to lyse the epiphytic bacterium Pantoea agglomerans. Representatives from the six molecular groups were studied by electron microscopy to determine their morphology. The phages exhibited distinct morphologies when examined by an electron microscope. Group I and 2 phages were tailed and contractile, and phages belonging to groups 3 to 6 had short tails or openings with thin appendages. Based on morphotypes, the bacteriophages of E. amylovora were placed in the order Caudovirales, in the families Myoviridae and Podoviridae.