Virulence of antibiotic-resistant Salmonella typhimurium
Virulence of antibiotic-resistant Salmonella typhimurium
复制标题
DOI：
10.1073/pnas.95.7.3949
复制
发表时间：
1998-03-31
影响因子：
11.1
通讯作者：
Andersson, DI
中科院分区：
文献类型：
--
作者：
Björkman, J;Hughes, D;Andersson, DI
我们发现，大多数对链霉素、利福平和萘啶酸耐药的鼠伤寒沙门氏菌突变体在小鼠中是无毒的，在所检测的7个耐药突变体中，SIS是无毒的，一个与野生型相似。在小鼠竞争实验中，耐药突变体迅速积累各种类型的补偿突变，恢复毒力而不伴随耐药性的丧失，这样的第二位点补偿突变比敏感野生型的回复更常见，我们从这些结果推断，减少抗生素的使用可能不会导致已经存在于人类和环境宿主中的耐药细菌的消失，因此，第二个位点的补偿性突变可以增加耐药细菌的适应性，使它们即使在无抗生素的环境中也能持续存在并成功地与敏感菌株竞争。
We show that most Salmonella typhimurium mutants resistant to streptomycin, rifampicin, and nalidixic acid are avirulent in mice, Of seven resistant mutants examined, sis were avirulent and one was similar to the wild type In competition experiments in mice, The avirulent-resistant mutants rapidly accumulated various types of compensatory mutations that restored virulence without concomitant loss of resistance, Such second-site compensatory mutations were more common then reversion to the sensitive wild type, We infer from these results that a reduction in the use of antibiotics might not result in the disappearance of the resistant bacteria already present in human and environmental reservoirs, Thus, second-site compensatory mutations could increase the fitness of resistant bacteria and allow them to persist and compete successfully with sensitive strains even in an antibiotic-free environment.