Human plasma carotenoid response to the ingestion of controlled diets high in fruits and vegetables
Human plasma carotenoid response to the ingestion of controlled diets high in fruits and vegetables
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DOI：
10.1093/ajcn/64.4.594
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发表时间：
1996-10-01
影响因子：
7.1
通讯作者：
Russell, RM
中科院分区：
文献类型：
--
作者：
Yeum, KJ;Booth, SL;Russell, RM
血浆类胡萝卜素的反应，测定在36名健康男性和女性之前和之后喂控制饮食与适量的脂肪（总能量的26%）和高类胡萝卜素含量（约16毫克/天）的两个15天的时期。此外，在交叉设计中，在第一个或第二个15-d停留期期间提供花椰菜（205 g/d）。血浆用脂肪酶和胆固醇酯酶消化，提取类胡萝卜素，用HPLC测定。使用C30类胡萝卜素柱分离了三种氧化类胡萝卜素（叶黄素、玉米黄质和隐黄质）、三种烃碳类胡萝卜素（α-胡萝卜素、全反式-β-胡萝卜素和13-顺式-β-胡萝卜素）以及番茄红素的四种几何异构体（15-顺式-、13-顺式-、9-顺式-和全反式-番茄红素）。与标准9-顺式-β-胡萝卜素共洗脱的一个小的未鉴定峰被鉴定为ζ-胡萝卜素（λ（max）= 400 nm）。高果蔬日粮显著提高了6-16日龄血浆叶黄素、隐黄质、α-胡萝卜素、13-顺式-β-胡萝卜素、全反式-β-胡萝卜素、顺式和反式-番茄红素的含量（P < 0.05）。提供额外的西兰花5 d的基础高类胡萝卜素饮食导致进一步显着增加血清中的叶黄素浓度与喂养的基础高类胡萝卜素饮食相比。大多数可测量的类胡萝卜素的人血浆中，可以增加在短时间内适度改变饮食，虽然血浆响应的幅度可能与基线caratenoid浓度。
Plasma carotenoid responses were determined in 36 healthy men and women before and after being fed controlled diets with a moderate amount of fat (26% of total energy) and a high carotenoid content (approximate to 16 mg/d) for two 15-d periods. in addition, broccoli (205 g/d) was provided either during the first or the second 15-d residency period in a crossover design. Plasma was digested with lipase and cholesterol esterase, and carotenoids were extracted and measured by using HPLC. Three oxygenated carotenoids (lutein, zeaxanthin, and cryptoxanthin), three hydrocarbon carbon carotenoids (alpha-carotene, all-trans-beta-carotene, and 13-cis-beta-carotene), and four geometric isomers of lycopene (15-cis-, 13-cis-, 9-cis-, and all-trans-lycopene) were separated by using a C30 carotenoid column. A small unidentified peak coeluted with standard 9-cis-beta-carotene and was identified as zeta-carotene (lambda(max) = 400 nm). The concentrations of plasma lutein, cryptoxanthin, alpha-carotene, 13-cis-beta-carotene, all-trans-beta-carotene, and cis- and trans-lycopenes were all significantly increased (P < 0.05) on days 6-16 by the high-fruit and -vegetable diets. The provision of additional broccoli for 5 d to the bas ic high-carotenoid diet resulted in a further significant increase in the serum concentration of lutein compared with the feeding of the basic high-carotenoid diet alone. Most of the measurable carotenoids of human plasma can be increased by moderate alterations in diet within a short time, although the magnitude of the plasma response may be related to the baseline caratenoid concentrations.