PLFAs of the microbial communities in composting mixtures of agro-industry sludge with different proportions of household waste
PLFAs of the microbial communities in composting mixtures of agro-industry sludge with different proportions of household waste
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DOI：
10.1016/j.ibiod.2010.01.012
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发表时间：
2010-10-01
影响因子：
4.8
通讯作者：
Hafidi, Mohamed
中科院分区：
文献类型：
--
作者：
Amir, Soumia;Abouelwafa, Rajae;Hafidi, Mohamed
在堆肥过程中的两个废物混合物富含脂肪M1（22%）和M2（39%）在不同的时间段的磷脂脂肪酸（PLFA）进行了分析。这两种混合物由从植物油精炼厂污水处理厂收集的污泥样品和生活垃圾组成。两种混合物的PLFA谱显示，在开始时，M2比M1更易产生真菌和革兰氏阴性菌（G-）M1的PLFA谱表现为非特异性G+和G-，M2的PLFA谱表现为非特异性G+和G-，M1的PLFA谱表现为非特异性G+和G-，M2的PLFA谱表现为非特异性G+和G-，M1的PLFA谱表现为非特异性G+和G-。在冷却阶段，细菌和真菌减少而在富含脂肪的M2中，Shannon-Weaver多样性指数（Ish）在M1堆肥过程中表现出更大的增加（0 69 ~ 1 05）主要为G+菌和G-菌，而M2堆肥中的G+菌和G-菌数量较少主成分分析和聚类分析结果表明，堆肥过程中真菌、G-细菌和G+细菌群落的变化趋势是连续的和嗜热和耐热的G-细菌堆肥结束的特点是减少所有这些微生物实体，特别是M1除了放线菌，这是与堆肥稳定性（C）2010爱思唯尔有限公司版权所有
Phospholipid fatty acids (PLFAs) were analysed at different time periods during composting of two waste mixtures rich in fats M1 (22%) and M2 (39%) with the aim of monitoring changes in microbial community structure The two mixtures consisted of a sludge sample collected from a vegetable oil refinery effluent treatment plant combined with household wastes The PLFA profiles of both mixtures revealed that at the start of the process fungi and Gram-negative bacteria (G-) were more abundant in M2 than in M1 During the thermophilic phase branched PLFA (i15 0 a15 0 i16 0 and i17 0) markers of Gram-positive bacteria (G+) became more abundant in M1 while G- bacteria were predominant in M2 The PLFA profiles in M1 representing non-specific G+ and G- bacteria as well as fungi decreased during the cooling phase (maturation) while an increase was recorded in M2 which was richer in fats The Shannon-Weaver diversity Index (Ish) showed a greater Increase during M1 composting (from 0 69 to 1 05) mainly for G+ bacteria and G- bacteria than in M2 composting (from 0 79 to 0 84) Principal components and cluster analyses revealed a succession of different communities during composting which varied from fungi and G- bacteria to G+ and thermophilic and thermotolerant G- bacteria The end of composting was characterized by a reduction of all these microbial entities especially for M1 except actinomycetes which are associated with compost stability (C) 2010 Elsevier Ltd All rights reserved