STUDIES ON CONFORMATIONAL PROPERTIES OF HIGH-MOBILITY-GROUP CHROMOSOMAL PROTEIN HMG 17 AND ITS INTERACTION WITH DNA
STUDIES ON CONFORMATIONAL PROPERTIES OF HIGH-MOBILITY-GROUP CHROMOSOMAL PROTEIN HMG 17 AND ITS INTERACTION WITH DNA
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DOI：
10.1111/j.1432-1033.1978.tb12154.x
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发表时间：
1978-01-01
期刊：
影响因子：
--
通讯作者：
JOHNS, EW
中科院分区：
文献类型：
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作者：
ABERCROMBIE, BD;KNEALE, GG;JOHNS, EW
[小牛胸腺]非组蛋白染色质蛋白，HMG 17，构象进行了研究，使用圆二色谱，红外光谱和核磁共振光谱，并通过小角散射。在游离溶液中，这种蛋白质几乎没有二级或三级结构，与其他高迁移率族蛋白HMG 1和2相反，它们具有高度有序的结构。蛋白HMG 17以离子依赖性方式与小牛胸腺DNA结合，以高蛋白/DNA比沉淀DNA。HMG 17的主要DNA结合区段可能是约15-40个残基之间的区段。
The conformation of the [calf thymus] non-histone chromatin protein, HMG 17, was studied using circular dichroism, IR and NMR spectroscopies, and by small-angle scattering. In free solution this protein has little or no secondary or tertiary structure, in contrast to the other high-mobility-group proteins, HMG 1 and 2, which exhibit highly ordered structures. Protein HMG 17 binds to calf thymus DNA in an ionic-dependent manner, precipitating the DNA at high protein/DNA ratio. The principle DNA-binding segment of HMG 17 is probably that between about residues 15-40.