Synthesis and application of biocompatible gold core-poly-(L-Lysine) shell nanoparticles
Synthesis and application of biocompatible gold core-poly-(L-Lysine) shell nanoparticles
复制标题
生物相容性金核聚赖氨酸壳纳米粒子的合成及应用
DOI：
10.1016/j.colsurfa.2016.07.014
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Li Lidong
中科院分区：
文献类型：
--
作者：
Wang Chun;Tang Fu;Wang Xiaoyu;Li Lidong
本文报道了一种荧光核壳纳米粒子的绿色合成方法。我们选择了一种常见的氨基酸色氨酸作为金核的绿色还原剂。然后使用生物大分子聚赖氨酸（l-赖氨酸）组装并交联到金纳米颗粒表面，作为生物相容性外壳。荧光增强后，观察到菁染料共轭到纳米颗粒表面。然后，将精氨酸-甘氨酸-天冬氨酸序列肽作为靶配体结合到壳上。细胞成像实验表明，制备的纳米颗粒对整合素阳性细胞的靶感测是有效的。此外，由于绿色合成方法，它们具有很高的生物相容性。因此，制备的纳米颗粒在细胞成像和靶向整合素检测方面具有很大的潜力。
In this work, green synthesis of a kind of fluorescent core-shell nanoparticles via a facile one-pot method was reported. We chose tryptophan, a common amino acid, to serve as the green reducing agent of the gold core. And a biomacromolecule, poly-(l-lysine), was then employed to assemble and cross-link onto gold nanoparticle surfaces serving as the biocompatible shell. Fluorescence enhancement was observed after a cyanine dye was conjugated onto the nanoparticle surfaces. Then, peptides with arginine-glycine-aspartic acid sequence were bound to the shell as targeting ligand. Cell imaging assay demonstrated that the prepared nanoparticles are efficient in target sensing of integrin-positive cells. Moreover, they possess high biocompatibility because of the green synthetic method. Thus, the prepared nanoparticles show great potential in cellular imaging and targeted integrin detection.