Structure and expression of glutathione S-transferase genes from the midgut of the Common cutworm, Spodoptera litura (Noctuidae) and their response to xenobiotic compounds and bacteria
Structure and expression of glutathione S-transferase genes from the midgut of the Common cutworm, Spodoptera litura (Noctuidae) and their response to xenobiotic compounds and bacteria
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斜纹夜蛾、斜纹夜蛾（夜蛾科）中肠谷胱甘肽 S-转移酶基因的结构和表达及其对外源化合物和细菌的反应
DOI：
10.1016/j.jinsphys.2011.05.001
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发表时间：
2011-07-01
影响因子：
2.2
通讯作者：
Zheng, Sichun
中科院分区：
文献类型：
--
作者：
Huang, Yufen;Xu, Zhibin;Zheng, Sichun
谷胱甘肽S-转移酶（GST）在细胞内内源性和外源性化合物的解毒和抗氧化应激中起着关键作用。本研究从斜纹夜蛾（Spodoptera litura）中肠中鉴定了5个GST基因，包括3个Sigma GST（SIGSTs 1、SIGSTs 2和SIGSTs 3）、1个Omega GST（SIGSTo 1）和1个未分类GST（SIGSTu 1）。对8个SIGSTs基因（包括先前鉴定的来自同一昆虫的Episodes GST基因、SIGSTe 1、SIGSTe 2和SIGSTe 3）的结构分析表明，Episodes SIGSTe基因不含任何内含子，而Sigma SIGSTs含有3个内含子，Omega SIGSTo 1和未分类的SIGSTu 1含有5个内含子。对这8个SIGSTs的时空表达分析表明，SIGSTe 1、SIGSTs 2和SIGSTo 1在从卵到成虫的各个发育阶段均有表达。SIGSTe 2、SIGSTe 3、SIGSTs 1、SIGSTs 3和SIGSTu 1在幼虫期的表达量高于其他各期，在中肠的表达量高于其他组织。SIGSTs 1在幼虫中肠中表达，但在脂肪体中不表达，并且可能由细菌感染诱导。毒死蜱不同程度地增加了SIGSTe 1、SIGSTe 3、SIGSTs 1和SIGSTs 3的表达，而黄毒素则增加了SIGSTe 1、SIGSTe 3、SIGSTs 1、SIGSTs 3和SIGSTo 1的表达。丙二醛的水平，氧化应激的指标，幼虫中肠比蛹中肠。毒死蜱能诱导幼虫体内丙二醛含量的增加，而黄毒素则无此作用。据推测，中肠SIGST的高表达水平可能是由于摄食、细菌感染和异生素化合物引起的氧化应激水平增加。(C)2011爱思唯尔有限公司版权所有。
Glutathione S-transferases (GSTs) play a pivotal role in detoxifying endogenous and xenobiotic compounds and oxidative stress resistance in cells. In this study, five GST genes, including three Sigma GSTs (SIGSTs1, SIGSTs2, and SIGSTs3), one Omega GST (SIGSTo1) and one un-classified GST (SIGSTu1)were identified from the midgut of the Common cutworm, Spodoptera litura. Structure analyses of the eight (including the previously identified Epsilon GST genes, SIGSTe1, SIGSTe2 and SIGSTe3 from the same insect) SIGSTs genes showed that the Epsilon SIGSTe genes do not contain any intron, while the Sigma SIGSTs contain three introns and the Omega SIGSTo1 and the un-classified SIGSTu1 contain five introns. Analysis of the spatial and temporal expression of these eight SIGSTs indicated that SIGSTe1, SIGSTs2 and SIGSTo1 expressed in all stages of development from the egg to the adult stages. SIGSTe2, SIGSTe3, SIGSTs1, SIGSTs3 and SIGSTu1 had higher expression levels in the larval stages than in other stages and their expression levels in the midgut were higher than in other tissues. SIGSTs1 was expressed in the larval midgut but not in the fat body and could be induced by bacterial infections. The expression of SIGSTe 1, SIGSTe3, SIGSTs1 and SIGSTs3 was increased by chlorpyrifos to various degrees, while the expression of SIGSTe1, SIGSTe3, SIGSTs1, SIGSTs3 and SIGSTo1 was increased by xanthotoxin. Levels of malonaldehyde, an indicator of oxidative stress, were higher in the larval midgut than in the pupal midgut. Chlorpyrifos induced the malonaldehyde content in the larvae, whereas xanthotoxin did not. It is hypothesized that high expression levels of the midgut SIGSTs might be due to the increased levels of oxidative stress caused by feeding, bacterial infection and xenobiotic compounds. (C) 2011 Elsevier Ltd. All rights reserved.