Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self‐Assembled Peptide Amphiphiles
Conformal Electrodeposition of Antimicrobial Hydrogels Formed by Self‐Assembled Peptide Amphiphiles
复制标题
DOI：
10.1002/admi.202300046
复制
发表时间：
2023-04
影响因子：
5.4
通讯作者：
Gervasio Zaldívar;Jiachen Feng;L. Lizarraga;Yafan Yu;L. D. de Campos;K. D. de Oliveira;K. Piepenbrink;Martin Conda-Sheridan;M. Tagliazucchi
中科院分区：
文献类型：
--
作者：
Gervasio Zaldívar;Jiachen Feng;L. Lizarraga;Yafan Yu;L. D. de Campos;K. D. de Oliveira;K. Piepenbrink;Martin Conda-Sheridan;M. Tagliazucchi
细菌生物膜在生物医学表面的定殖令人担忧，因为这些微生物在生物膜中比在液体培养物中表现出更高的抗菌耐药性。开发可轻松应用于医学相关复杂形状物体（例如植入物和手术器械）的抗菌涂层是一个重要且具有挑战性的研究方向。这项工作报告了通过电沉积自组装阳离子肽两亲物（PA）的保形水凝胶来制备抗菌表面。电沉积三种PA的水凝胶：C16K2、C16K3和C18K2，其中Cn是n个亚甲基的烷基链，Km是m个赖氨酸的寡肽。处理变量（电沉积时间、电势、pH、盐浓度、搅拌）能够精细控制薄膜厚度，展示了该方法的灵活性，并能够揭示电沉积的机制。电化学制备的水凝胶可抑制琼脂平板中金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的生长，并阻止鲍曼不动杆菌和铜绿假单胞菌生物膜的形成以及固体培养基中鲍曼不动杆菌菌落的形成。 C16K2和C16K3水凝胶的抗菌活性优于C18K2，同时保持与人体细胞良好的相容性。
The colonization of biomedical surfaces by bacterial biofilms is concerning because these microorganisms display higher antimicrobial resistance in biofilms than in liquid cultures. Developing antimicrobial coatings that can be easily applied to medically‐relevant complex‐shaped objects, such as implants and surgical instruments, is an important and challenging research direction. This work reports the preparation of antibacterial surfaces via the electrodeposition of a conformal hydrogel of self‐assembling cationic peptide‐amphiphiles (PAs). Hydrogels of three PAs are electrodeposited: C16K2, C16K3, and C18K2, where Cn is an alkyl chain of n methylene groups and Km is an oligopeptide of m lysines. The processing variables (electrodeposition time, potential, pH, salt concentration, agitation) enable fine control of film thickness, demonstrating the flexibility of the method and allowing to unravel the mechanisms underlying electrodeposition. The electrochemically prepared hydrogels inhibit the growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa in agar plates, and prevent the formation of biofilms of Acinetobacter baumannii and P. aeruginosa and the formation of A. baumannii colonies in solid media. C16K2 and C16K3 hydrogels outperform the antimicrobial activity of those of C18K2 while maintaining good compatibility with human cells.