Inhibition of Dugbe nairovirus replication by human MxA protein
Inhibition of Dugbe nairovirus replication by human MxA protein
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DOI：
10.1016/j.virusres.2003.10.002
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发表时间：
2004-01-01
期刊：
影响因子：
5
通讯作者：
Elliott, RM
中科院分区：
文献类型：
--
作者：
Bridgen, A;Dalrymple, DA;Elliott, RM
先前已证明属于布尼亚病毒科的正布尼亚病毒属、汉坦病毒属和白蛉病毒属的病毒对干扰素诱导蛋白 MxA 具有敏感性。我们将这些发现扩展到第四个也是剩下的包含感染人类和其他动物的病毒属的成员，即内罗病毒杜格贝病毒（DUGV）。使用 VA9 细胞（永久转染 MxA cDNA 的 Vero 细胞）进行的间接免疫荧光实验显示，DUGV 抗原表达大幅降低，表明 MxA 抑制 DUGV 复制。 Western 和 Northern 印迹分析显示，在 MxA 存在的情况下，DUGV 核衣壳 (N) 蛋白表达和 DUGV 基因组 RNA 分别显着降低。与对照 VN36 细胞相比，VA9 细胞中的病毒滴度也降低了两个数量级以上。这一发现可能适用于内罗病毒治疗。 (C) 2003 Elsevier B.V. 保留所有权利。
Sensitivity to the interferon-induced protein, MxA, has previously been demonstrated for viruses belonging to the Orthobunyavirus, Hantavirus and Phlebovirus genera of the Bunyaviridae family. We have extended these findings to a member of the fourth and remaining genus containing viruses that infect man and other animals, the nairovirus Dugbe virus (DUGV). Indirect immunofluorescence experiments using VA9 cells (Vero cells permanently transfected with MxA cDNA) revealed strongly reduced DUGV antigen expression, suggesting that MxA inhibited DUGV replication. Western and Northern blot analyses showed significantly lower DUGV nucleocapsid (N) protein expression and DUGV genomic RNA, respectively, in the presence of MxA. Viral titres were also reduced by more than two orders of magnitude in VA9 cells compared with control VN36 cells. This finding may have application to nairovirus therapeutics. (C) 2003 Elsevier B.V. All rights reserved.