Fishing out Methionine-Containing Proteins from Complex Biological Systems Based on a Non-Enzymatic Biochemical Reaction
Fishing out Methionine-Containing Proteins from Complex Biological Systems Based on a Non-Enzymatic Biochemical Reaction
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基于非酶生化反应从复杂生物系统中提取含蛋氨酸的蛋白质
DOI：
10.1021/acs.nanolett.0c03535
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发表时间：
2021
期刊：
影响因子：
10.8
通讯作者：
Tang Bo
中科院分区：
文献类型：
--
作者：
Luan Dongrui;Zheng Aishan;Gao Xiaonan;Xu Kehua;Tang Bo
发展一种简单、快速、低毒的从复杂生物体系中鉴定和分离蛋白质的方法仍然是一个巨大的挑战。在此基础上，设计并合成了一种纳米复合材料(Fe3O4@Au-Se多肽)，用于在HOBr存在下将赖氨酸的氨基和蛋氨酸的甲基偶联，从而筛选出含有蛋氨酸的蛋白质。将含有4个赖氨酸残基(Lys-{Se-Cys})的多肽连接到Fe3O4@Au纳米复合材料上，通过S═N交联有效地捕获蛋氨酸残基。通过磁选获得了含蛋氨酸的蛋白质，并在H_2Se的影响下从Fe_3O_4@Au-Se-多肽纳米复合材料中释放出来。HRMS和SDS-PAGE结果证实，蛋氨酸蛋白可以成功地从混合液中分离出来。这项工作为从复杂生物系统中识别和分离一类蛋白质提供了一种有用的策略。
Nowadays, it still remains a great challenge to develop a simple, fast, and low-toxic method for identification and separation of proteins from complex biological systems. Herein, a nanocomposite (Fe3O4@Au–Se–peptide) was designed and synthesized to fish out methionine-containing proteins based on a non-enzymatic biochemical reaction, which couples amino groups of lysine with theS-methyl group of methionine in the presence of HOBr. Peptides which contain four lysine residues (Lys-Lys-Lys-Lys-{Se-Cys}) linked to the Fe3O4@Au nanocomposites were used to capture methionine residues efficientlyviaa S═N cross-linking. The methionine-containing protein was obtained by magnetic separation and released from the Fe3O4@Au–Se–peptide nanocomposites with the influence of H2Se. The HRMS and SDS-PAGE results confirmed the methionine-containing protein could be successfully fished out from a mixture solution. This work provides a useful strategy for recognition and separation of a category of proteins from complex biological systems.