High-affinity reactions between antigen-specific T-cell receptors and peptides associated with allogeneic and syngeneic major histocompatibility complex class I proteins.
High-affinity reactions between antigen-specific T-cell receptors and peptides associated with allogeneic and syngeneic major histocompatibility complex class I proteins.
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抗原特异性 T 细胞受体与同种异体和同系主要组织相容性复合物 I 类蛋白相关肽之间的高亲和力反应。
DOI：
10.1073/pnas.91.24.11487
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发表时间：
1994
影响因子：
11.1
通讯作者：
Eisen,HN
中科院分区：
文献类型：
--
作者：
Sykulev,Y;Brunmark,A;Tsomides,TJ;Kageyama,S;Jackson,M;Peterson,PA;Eisen,HN
我们在这里报告的抗原特异性T细胞受体（TCR）的两个不相关的CD 8 + T细胞克隆各自的肽-主要组织相容性复合物（MHC）配体的内在亲和力高于一般认为占上风的TCR值。一个克隆（2C）的TCR结合与α-酮戊二酸脱氢酶九肽（QLSPFPFDL，称为QL 9）缔合的同种异体I类MHC蛋白（Ld），其固有亲和力（固有平衡缔合常数）为1-2 × 10（7）M-1。另一个克隆（4G 3）的TCR以1.5 × 10（6）M-1的固有亲和力结合与卵白蛋白八肽（SIINFEKL，称为pOV 8）缔合的同基因I类MHC蛋白（Kb）。两个克隆的比较，结合目前的观点，T细胞库的选择在胸腺中，导致我们提出，TCR亲和力一般可能是更高的同种异体MHC-肽复合物比同基因MHC-肽复合物。
We report here that the intrinsic affinities of the antigen-specific T-cell receptors (TCR) of two unrelated CD8+ T-cell clones for their respective peptide-major histocompatibility complex (MHC) ligands are higher than the values generally thought to prevail for TCR. The TCR of one clone (2C) binds an allogeneic class I MHC protein (Ld) in association with an alpha-ketoglutarate dehydrogenase nonapeptide (QLSPFPFDL, termed QL9) with an intrinsic affinity (intrinsic equilibrium association constant) of 1-2 x 10(7) M-1. The TCR of the other clone (4G3) binds a syngeneic class I MHC protein (Kb) in association with an ovalbumin octapeptide (SIINFEKL, termed pOV8) with an intrinsic affinity of 1.5 x 10(6) M-1. A comparison of the two clones, combined with current views of T-cell repertoire selection in the thymus, leads us to propose that TCR affinities are generally likely to be higher for allogeneic MHC-peptide complexes than for syngeneic MHC-peptide complexes.