Beneficial effect of GB virus C co-infection in human immunodeficiency virus type 1-infected individuals
Beneficial effect of GB virus C co-infection in human immunodeficiency virus type 1-infected individuals
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DOI：
10.1111/j.1348-0421.2007.tb03901.x
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Kaneda, Tsuguhiro
中科院分区：
文献类型：
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作者：
Hattori, Junko;Okumura, Naoya;Kaneda, Tsuguhiro
几份报告记录了合并感染GBV-C的HIV-1感染患者的预后较好，而其他报告与这些发现相矛盾，结果是这一问题仍然存在争议。我们试图阐明GBV-C混合感染对HIV-1感染者影响的复杂状况。RT/巢式聚合酶链式反应检测182例HIV-1感染者(20.3%)中有37例GBV-C RNA阳性。实时荧光定量检测的GBV-C病毒载量在7.8×10(3)~3.3×10(6)拷贝/ml之间，19例HAART初治患者GBV-C载量与HIV-1病毒载量呈弱负相关，提示GBV-C载量较高与HIV-1复制受到较大抑制有关。先前发表的一项体外研究表明，GBV-C感染会诱导RANTES上调，导致HIV-1复制受到抑制。然而，在我们目前的研究中，GBV-C混合感染组的血液RANTES水平显著低于未感染组(190-9,959对264-31,038 pg/ml，P=0.004)。我们的结果表明，GBV-C联合感染对HIV-1复制的抑制不是由上调的RANTES介导的，因此需要另一个未知因素。
Several reports have documented a better prognosis for HIV-1-infected patients co-infected with GBV-C, while other reports have contradicted such findings with the result that this issue remains controversial. We attempted to clarify the complicated status of the effect of GBV-C co-infection on HIV-1-infected patients. GBV-C RNA was detected in 37 samples in 182 HIV-1-infected patients (20.3%) using RT/nested PCR. Of these, 3 were determined to be GBV-C genotype 1, 12 were genotype 2, and the remaining 22 were genotype 3. The GBV-C viral load quantified by real-time PCR ranged from 7.8 x 10(3) to 3.3 x 10(6) copies/ml. Weakly negative correlation was observed between GBV-C viral load and HIV-1 viral load in 19 HAART-naive patients, indicating that a higher GBV-C viral load is associated with a greater suppression of HIV-1 replication. A previously published in vitro study suggested that GBV-C infection would induce up-regulation of RANTES, leading to suppression of HIV-1 replication. However, in our present study, the blood RANTES level was significantly lower in the GBV-C co-infected group than in the uninfected group (190-9,959 vs. 264-31,038 pg/ml, P=0.004). Our results suggested that a suppression of HIV-1 replication by GBV-C co-infection is not mediated by up-regulated RANTES, and thus call for another as yet unknown factor.