Forced swim test-induced neurochemical, endocrine, and immune changes in the rat
Forced swim test-induced neurochemical, endocrine, and immune changes in the rat
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DOI：
10.1016/s0091-3057(97)00028-2
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发表时间：
1997-12-01
影响因子：
3.6
通讯作者：
Leonard, BE
中科院分区：
文献类型：
--
作者：
Connor, TJ;Kelly, JP;Leonard, BE
强迫游泳试验（FST）是一种广泛用作啮齿动物抗抑郁活性筛选试验的行为范式。本研究的目的是表征皮质酮和免疫反应，除了检查神经递质水平，在五个大脑区域的间隔（15，30，60，90和120分钟）后，第二次暴露于FST。有一个显着的，但短暂的减少去甲肾上腺素和5-HT浓度，在下丘脑15分钟后FST曝光。FST暴露后20-120 min，额叶皮质和杏仁核中5-HT的周转率显著增加。FST引起了一个强大的皮质酮反应，在30分钟达到峰值显着，并几乎恢复到基线后120分钟曝光。FST暴露后120 min，总白色血细胞计数显著减少，同时FST暴露后90和120 min淋巴细胞百分比显著减少。此外，有一个显着的，但短暂的抑制PHA和Con A诱导的淋巴细胞增殖15分钟后FST曝光。这项研究表明，有神经化学变化，是一致的内分泌和免疫变化与FST暴露在大鼠。此外，该模型可用于检查抗抑郁药物对这种应激反应的操纵效果。这样的研究可以增加我们对抗抑郁药，压力和神经内分泌和免疫系统之间相互作用的理解。(C)1997年爱思唯尔科学公司
The forced swim test (FST) is a behavioral paradigm that is widely used as a screening test for antidepressant activity in rodents. The objectives of the present study were to characterize the corticosterone and immune responses and in addition to examine neurotransmitter levels, in five brain regions at intervals (15, 30, 60, 90, and 120 min) following the second exposure to the FST. There was a significant but transient reduction in noradrenaline and 5-HT concentrations, in the hypothalamus 15 min post-FST exposure. 5-HT turnover in the frontal cortex and amygdala was significantly increased between 20-120 min post-FST exposure. The FST elicited a robust corticosterone response that peaked significantly at 30 min and had almost returned to baseline 120 min after exposure. There was a significant reduction in total white blood cell count 120 min after the FST, which was accompanied by a sig nificantly reduced percentage of lymphocytes 90 and 120 min post-FST exposure. In addition, there was a significant but transient suppression of both PHA and Con A-induced lymphocyte proliferation 15 min following FST exposure. This study demonstrates that there are neurochemical changes that are coincident with the endocrine and immune changes associated with FST exposure in rats. Furthermore, this model could be used to examine the effects of manipulation of this stress response by antidepressant drugs. Such an investigation could add to our understanding of the interactions between antidepressants,stress and the neuroendocrine and immune systems. (C) 1997 Elsevier Science Inc.