FGF-20, a novel neurotrophic factor, preferentially expressed in the substantia nigra pars compacta of rat brain
FGF-20, a novel neurotrophic factor, preferentially expressed in the substantia nigra pars compacta of rat brain
复制标题
DOI：
10.1006/bbrc.2000.3675
复制
发表时间：
2000-10-22
影响因子：
3.1
通讯作者：
Itoh, N
中科院分区：
文献类型：
--
作者：
Ohmachi, S;Watanabe, Y;Itoh, N
我们已经从大鼠脑中分离出编码一种新的FGF（212个氨基酸）的cDNA。因为这是FGF家族的第20个有记载的成员，我们暂时将其称为FGF-20。在FGF家族成员中，FGF-20与FGF-9和FGF-16最相似（分别为70%和62%的氨基酸同一性）。人FGF-20基因定位于人基因组8p21.3-p22区域。人FGF-20与大鼠FGF-20高度相同（95%氨基酸同一性）。FGF-20 mRNA在成年大鼠主要组织中优先表达。用原位杂交法检测了FGF-20 mRNA在大鼠脑内的定位。FGF-20 mRNA优先表达于黑质丘脑部。为了检测FGF-20的生物学活性，用含有大鼠FGF-20 cDNA的重组杆状病毒感染昆虫细胞，产生重组大鼠FGF-20。重组大鼠FGF-20增强中脑多巴胺能神经元的存活。本研究结果表明，FGF-20是一种新的神经营养因子，优先表达于大鼠脑黑质致密体。(C)北京大学出版社.
We have isolated cDNA encoding a novel FGF (212 amino acids) from rat brain. Because this is the 20th documented member of the FGF family, we tentatively term it FGF-20, Among FGF family members, FGF-20 is most similar to FGF-9 and FGF-16 (70 and 62% amino acid identity, respectively). Human FGF-20 gene was found in the human genomic sequence mapped to the 8p21.3-p22 region. Human FGF-20 is highly identical to rat FGF-20 (95% amino acid identity). FGF-20 mRNA was preferentially expressed in rat brain among the adult major tissues examined. The localization of FGF-20 mRNA in rat brain was also examined by in situ hybridization. FGF-20 mRNA was preferentially expressed in the substantia nigra pars compacta. To examine the biological activity of FGF-20, recombinant rat FGF-20 was produced by insect cells infected with recombinant baculovirus containing rat FGF-20 cDNA, Recombinant rat FGF-20 enhanced the survival of midbrain dopaminergic neurons. The present results indicate that FGF-20 is a novel neurotrophic factor preferentially expressed in the substantia nigra pars compacts of rat brain. (C) 2000 Academic Press.