Attempt to Harvest a Sufficient Number of Mononuclear Cells in an Appropriate Blood Product Volume By Modification of the Default Apheresis Setting.
Attempt to Harvest a Sufficient Number of Mononuclear Cells in an Appropriate Blood Product Volume By Modification of the Default Apheresis Setting.
复制标题
尝试通过修改默认单采设置来在适当的血液产品体积中收获足够数量的单核细胞。
DOI：
10.1111/1744-9987.12562
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Katayama N
中科院分区：
文献类型：
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作者：
Tanaka Y;Ohishi K;Sawai T;Iwasaki H;Kageyama S;Masuya M;Matsumoto T;Tanigawa T;Wada H;Shiku H;Ito M;Katayama N
为了采集T细胞治疗，在COBE Spectra细胞分离器中，采用MNC模式（N =10）采集的CD3+T细胞数量是Auto PBSC模式（N =5）的1.6倍，但血液制品体积增加了3.5倍。对于治疗性血管生成治疗，最初使用Auto PBSC模式（N =4）进行单采，以微调血液制品体积以忽略细胞浓度，但收集的单核细胞数量低于预期。然而，收获循环数从3.8 ± 0.5增加至7.4 ± 2.0个循环（N =19），导致采集的单核细胞数量增加2.1倍（8.7 ± 4.1 × 109 vs. 4.1± 1.0 × 109细胞，P <0.05）。通过这种修改增加的血液制品体积似乎低于MNC模式的预期。这些数据表明，通过修改默认收集设置可以实现最佳收获。
To harvest for T cell therapy, a 1.6‐fold higher number of CD3+T cells was collected with MNC mode (N =10) compared with Auto PBSC mode (N =5) in COBE Spectra cell separator, but the blood product volume was increased by 3.5‐fold. For therapeutic angiogenesis therapy, apheresis was initially performed using Auto PBSC mode (N =4) to fine tune the blood product volume to omit cell concentration, but the collected number of mononuclear cells was lower than expected. However, an increase of the harvest cycle number from 3.8 ± 0.5 to 7.4 ± 2.0 cycles (N =19) resulted in a 2.1‐fold higher number of collected mononuclear cells (8.7 ± 4.1 × 109vs. 4.1 ± 1.0 × 109cells,P <0.05). The increase in blood product volume by this modification appeared to be lower than that expected with MNC mode. These data show that optimal harvesting can be achieved by modification of default collection settings.