Effects of Keishi-bukuryo-gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats
Effects of Keishi-bukuryo-gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats
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DOI：
10.1078/0944-7113-00336
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发表时间：
2004-02-01
期刊：
影响因子：
7.9
通讯作者：
Terasawa, K
中科院分区：
文献类型：
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作者：
Goto, H;Shimada, Y;Terasawa, K
Keishi-bukuryo-gan（桂枝茯苓丸）是一种用于改善血液循环的配方。最近它也经常被用于治疗动脉硬化。所涉及的机制之一被认为是改善内皮功能障碍，但细节仍不清楚。在本研究中，研究了 Keishi-bukuryo-gan 对自发性糖尿病 (WBN/kob) 大鼠血管功能和血液流变学因素的影响。给大鼠喂食 Keishi-bukuryo-gan 30 周。测量体重、血糖、内皮依赖性/非依赖性舒张、自由基诱导的血管收缩和收缩性前列腺素、甘油三酯、晚期糖基化终产物、脂质过氧化物、血清NO2-/NO3-和血液粘度。结果表明，Keishi-bukuryo-gan 导致乙酰胆碱引起的内皮依赖性舒张的减少显着增加，自由基和收缩性前列腺素引起的血管收缩显着减少。此外，血清NO2-/NO3-和血液粘度显着降低。根据这些结果，推测Keishi-bukuryo-gan对内皮发挥了保护作用。 WBN/kob 大鼠是人类糖尿病并发症的有用研究模型，Keishi-bukuryo-gan 在易感大鼠中显示出对血管损伤的保护作用。
Keishi-bukuryo-gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-bukuryo-gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-bukuryo-gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation end-product, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-bukuryo-gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-bukuryo-gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-bukuryo-gan showed a protective effect against vascular injury in the susceptible rat.