Human microvascular endothelial cell-extracellular matrix interaction in cellular growth state determination.
Human microvascular endothelial cell-extracellular matrix interaction in cellular growth state determination.
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人微血管内皮细胞-细胞外基质相互作用在细胞生长状态测定中的作用。
DOI：
10.1007/bf00300689
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发表时间：
1995
影响因子：
3.6
通讯作者：
Stephens,RE
中科院分区：
文献类型：
--
作者：
Mallery,SR;Lantry,LE;Toms,MC;Titterington,LC;Hout,BL;Brierley,GP;Stephens,RE
我们介绍两种方法，这两种方法都是基于细胞-细胞外基质的相互作用，这将有助于人类微血管内皮细胞的研究。一种方法描述了在人微血管内皮细胞中获得G1群体基线的方法。由于细胞外基质在内皮细胞生长中的贡献，只有在将血管生成抑制水平的肝素和氢化可的松掺入细胞外基质后，G1中的同步才有可能。在第二种方法中，我们证明了人微血管内皮细胞-细胞外基质相互作用的选择性扰动导致在人微血管内皮细胞中诱导增殖和分化生长状态之间的过渡生长状态。在功能性微管形成测定中，过渡生长状态内皮细胞显示的速率介于从分化和增殖内皮细胞获得的速率之间。我们的研究结果表明，人微血管内皮细胞-细胞外基质的相互作用在确定细胞生长状态的重要性。我们的研究结果还表明，微血管内皮细胞的细胞外基质环境的反应是最高的分化生长状态。
We introduce two methods, both of which are based on cellular-extracellular matrix interaction, which will facilitate the study of human microvascular endothelial cells. One method describes the means to obtain a G1 population baseline in human microvasclular endothelial cells. Because of the contribution of the extracellular matrix in endothelial cell growth, synchronization in G1 was possible only after the incorporation of angiostatic levels of heparin and hydrocortisone into the extracellular matrix. In the second method, we demonstrate that selective perturbation of human microvascular endothelial cell-extracellular matrix interactions results in the induction of a transitional growth state, between proliferative and differentiated growth states, in human microvascular endothelial cells. In the functional, microtubule formation assays, transitional growth state endothelial cells display rates that are indermediate between those obtained from differentiated and proliferative endothelial cells. Our results demonstrate the importance of the human microvascular endothelial cell-extracellular matrix interaction in the determination of cellular growth state. Our findings also imply that responsiveness of microvascular endothelial cells to their cellular-extracellular matrix environs is highest during the differentiated growth state.