CCR7 and CXCR4 expression predicts lymph node status including micrometastasis in gastric cancer
CCR7 and CXCR4 expression predicts lymph node status including micrometastasis in gastric cancer
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DOI：
10.3892/ijo_00000308
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发表时间：
2009-07-01
影响因子：
5.2
通讯作者：
Aikou, Takashi
中科院分区：
文献类型：
--
作者：
Arigami, Takaaki;Natsugoe, Shoji;Aikou, Takashi
趋化因子受体CCR 7和CXCR 4在原发肿瘤细胞的淋巴结转移机制中起主要作用。我们推测它们在胃肿瘤细胞中的表达可以预测淋巴结状态，包括淋巴结微转移（LNMM）。我们用免疫组化方法检测了93例胃肿瘤切除标本中CCR 7和CXCR 4的表达。通过逆转录-聚合酶链反应和免疫组织化学使用细胞角蛋白单克隆抗体检测LNMM，以及苏木精-伊红（H&E）染色来检查解剖的淋巴结。H&E染色结果显示，CCR 7和CXCR 4分别在26.9%（25/93）和32.3%（30/93）的肿瘤细胞中高表达，且与淋巴结转移显著相关（P=0.0212和P=0.0115）。我们在83例淋巴结阴性患者中发现了25例（30.1%）LNMM。CCR 7和CXCR 4的表达均与淋巴结状态（包括LNMM）显著相关（分别为P=0.0092和P=0.0075）。此外，CCR 7和CXCR 4的联合表达水平与淋巴结转移状态显著相关（P=0.0021）。评估CCR 7和CXCR 4在胃癌中的表达是预测包括LNMM在内的淋巴结转移状态的有用工具。
The chemokine receptors CCR7 and CXCR4 play a major role in the mechanism of lymph node metastasis from primary tumor cells. We postulated that their expression in gastric tumor cells could predict lymph node status including lymph node micrometastasis (LNMM). We assessed CCR7 and CXCR4 expression in 93 resected gastric tumor specimens by immunohistochemistry. Dissected lymph nodes were examined by reverse transcription-polymerase chain reaction and immunohistochemistry using cytokeratin monoclonal antibody to detect LNMM in addition to hematoxylin-eosin (H&E) staining. Levels of CCR7 and CXCR4 expression were high in 26.9% (25/93) and in 32.3% (30/93), respectively of tumor cells and the levels significantly correlated with lymph node metastasis according to H&E staining (P=0.0212 and P=0.0115, respectively). We identified LNMM in 25 of 83 (30.1%) node-negative patients. Both CCR7 and CXCR4 expression significantly correlated with lymph node status including LNMM (P=0.0092 and P=0.0075, respectively). Furthermore, levels of combined CCR7 and CXCR4 expression significantly correlated with lymph node metastatic status (P=0.0021). Assessment of CCR7 and CXCR4 expression in gastric cancer is a useful tool for predicting lymph node metastatic status including LNMM.