Effect of ultrasound irradiation on α-SMA and TGF-β1 expression in human dermal fibroblasts.
Effect of ultrasound irradiation on α-SMA and TGF-β1 expression in human dermal fibroblasts.
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发表时间：
2011
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通讯作者：
N. Maeshige;H. Terashi;Michiko Aoyama;Kazuhiro Torii;M. Sugimoto;M. Usami
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作者：
N. Maeshige;H. Terashi;Michiko Aoyama;Kazuhiro Torii;M. Sugimoto;M. Usami
超声治疗是一种促进压疮愈合的辅助治疗方法。然而，这种治疗的有效性和科学依据尚不清楚。我们研究了超声辐射对人真皮成纤维细胞α-平滑肌肌动蛋白（α-SMA）和转化生长因子β 1（TGF-β1）表达的影响。这些是加速伤口闭合的重要因素。我们使用0、0.1、0.5和1.0 W/cm 2的脉冲超声。实时荧光定量PCR检测TGF-β1和α-SMA mRNA的表达，Western blot检测α-SMA蛋白的表达，免疫荧光染色检测α-SMA的定位。α-SMA和TGF-β1 mRNA表达在照射后24 h增加，48 h无明显变化。0 W/cm²和0.1 W/cm²的对照组之间存在显著差异，α-SMA增加1.34 ± 0.26倍（P < 0.05），TGF-β1增加1.78 ± 0.57倍（P < 0.05）。超声辐照24 h后，成纤维细胞中α-SMA蛋白表达增加。超声辐射促进人皮肤成纤维细胞α-SMA表达，提示超声治疗慢性创伤的生物学机制。
Ultrasound therapy is used to promote pressure ulcer healing as an adjunctive therapy. However, the efficacy and the scientific basis of this treatment are unclear. We investigated the effect of ultrasound irradiation on alpha-smooth muscle actin (α-SMA) and transforming growth factor-beta1 (TGF-β1) expression in human dermal fibroblasts. These are important factors for acceleration of wound closure. We used pulsed ultrasound of 0, 0.1, 0.5, and 1.0 W/cm2. TGF-β1 and α-SMA mRNA was measured by quantitative real-time polymerase chain reaction, α-SMA protein was examined by western blot, and localization of α-SMA was evaluated by immunofluorescence staining. Expression of α-SMA and TGF-β1 mRNA was increased at 24 h but not at 48 h after ultrasound irradiation. There were significant differences between controls of 0 W/cm² and 0.1 W/cm² with a 1.34 ± 0.26 fold increase in α-SMA (P < 0.05) and a 1.78 ± 0.57 fold increase in TGF-β1 (P < 0.05). Protein levels of α-SMA were also increased and detected in ultrasound irradiated fibroblasts at 24 h. Ultrasound irradiation promotes α-SMA expression in human dermal fibroblasts and this suggests the biological mechanism of ultrasound efficacy on chronic wound treatment.