Rapid turnover of FlhD and FlhC, the flagellar regulon transcriptional activator proteins, during Proteus swarming
Rapid turnover of FlhD and FlhC, the flagellar regulon transcriptional activator proteins, during Proteus swarming
复制标题
DOI：
10.1128/jb.182.3.833-836.2000
复制
发表时间：
2000-02-01
影响因子：
3.2
通讯作者：
Hughes, C
中科院分区：
文献类型：
--
作者：
Claret, L;Hughes, C
肠细菌flhDC主操纵子激活鞭毛生物发生基因层级的表达并且还抑制细胞分裂。在奇异变形杆菌分化成伸长的超鞭毛群细胞期间，flhDC转录强烈但短暂地增加。我们发现，FlhD和FlhC蛋白的浓度也严格控制在翻译后水平。这是通过蛋白质降解来实现的，当两种蛋白质的半衰期约为时，分化后的蛋白质降解最为严重。降解是能量依赖性的，并且降解涉及Lon蛋白酶。
The enterobacterial flhDC master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. During Proteus mirabilis differentiation into elongated hyperflagellated swarm cells, flhDC transcription is strongly but transiently increased. We show that concentration of the FlhD and FlhC proteins is also tightly controlled at the posttranslational level. This is achieved by protein degradation, which is most severe after differentiation when the half-life of both proteins is ca. 2 min. Degradation is energy dependent and putatively involves the Lon protease.