Long-term culture and functionality of pancreatic islets monitored using microelectrode arrays.
Long-term culture and functionality of pancreatic islets monitored using microelectrode arrays.
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使用微电极阵列监测胰岛的长期培养和功能
DOI：
10.1039/c3ib40261d
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Krippeit-Drews
中科院分区：
文献类型：
--
作者：
Schönecker;Kraushaar;Düfer;Härdtner;Guenther;Walther;Lendeckel;Barthlen;Krippeit-Drews
在微电极阵列 (MEA) 上对小鼠胰岛葡萄糖诱导的电活动进行细胞外记录，是解决 β 细胞（病理）生理学问题的创新且强大的工具。在双重方法中，我们测试了该技术是否可以检测浓度依赖性药物效应以及表征长期培养期间β细胞活性的变化。首先，我们建立了通过记录电活动来长期研究 β 细胞功能的条件。该结果首次测量了长期培养过程中个体小鼠胰岛的 β 细胞膜电位振荡。胰岛分离后记录振荡长达 34 天。重要的是，电活动对葡萄糖的依赖性在一个月内没有变化。因此，我们可以在数周内跟踪由 β 细胞环境变化引起的单个胰岛的电生理变化。其次，我们使用 MEA 技术来测定氧化应激引起的 β 细胞损伤并评估适当的保护机制。氧化应激在 2 型糖尿病 (T2DM) 的发展中起着关键作用。 H2O2 对电活动的急性影响的检查表明，氧化剂以浓度依赖性方式降低电活动。超氧化物歧化酶模拟物 tempol 可防止 H2O2 的有害影响。总之，我们证明 MEA 记录可用于解决 β 细胞的疾病相关机制和保护性干预措施。未来，这项基础工作应该能够在受控的体外条件下监测朗格汉斯岛的电活动，包括长期暴露于氧化应激、糖脂毒性和其他糖尿病诱导剂。
Extracellular recording of the glucose-induced electrical activity of mouse islets of Langerhans on microelectrode arrays (MEAs) is an innovative and powerful tool to address beta-cell (patho-)physiology. In a dual approach we tested whether this technique can detect concentration-dependent drug effects as well as characterize alterations in beta-cell activity during prolonged culture. First we established conditions that allow long-term investigation of beta-cell function by recording electrical activity. The results provide the first measurements of beta-cell membrane potential oscillations of individual murine islets during long-term culture. Oscillations were recorded for up to 34 days after islet isolation. Importantly, the glucose dependence of electrical activity did not change over a period of one month. Thus we can follow electrophysiological changes of individual islets induced by alterations in the beta-cell environment over weeks. Second, we used the MEA technique to assay beta-cell damage induced by oxidative stress and to evaluate appropriate protection mechanisms. Oxidative stress plays a key role in the development of type 2 diabetes mellitus (T2DM). Examination of the acute effects of H2O2on electrical activity showed that the oxidant reduced the electrical activity in a concentration-dependent manner. The superoxide dismutase mimetic, tempol, protected against the detrimental effects of H2O2. In conclusion, we demonstrated that MEA recordings can be used to address disease-related mechanisms and protective interventions in beta-cells. In the future, this fundamental work should enable the monitoring of the electrical activity of islets of Langerhans under controlledex vivoconditions including long-term exposure to oxidative stress, glucolipotoxicity, and other diabetes-inducing agents.
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影响因子：
6.6
作者：
Chu, Kwan Yi;Leung, Po Sing
通讯作者：
Leung, Po Sing
影响因子：
4.3
作者：
Zorov, Dmitry B.;Juhaszova, Magdalena;Sollott, Steven J.
通讯作者：
Sollott, Steven J.
影响因子：
4.8
作者：
Herson, PS;Lee, K;Ashford, MLJ
通讯作者：
Ashford, MLJ
通过微电极阵列在细胞外记录膜电位振荡来快速评估 β 细胞的功能
DOI：
--
发表时间：
2011
期刊：
Pflügers Archiv: European Journal of Physiology
影响因子：
--
作者：
T. Pfeiffer;U. Kraushaar;M. Düfer;S. Schönecker;D. Haspel;E. Günther;G. Drews;P. Krippeit
通讯作者：
P. Krippeit
ATP 敏感性 K+ 通道参与自由基介导的大鼠胰腺 β 细胞胰岛素分泌抑制
影响因子：
7.7
作者：
M. Nakazaki;M. Kakei;N. Koriyama;Hiromitsu Tanaka
通讯作者：
Hiromitsu Tanaka