Complete mapping of glomeruli based on sensory nerve branching pattern in the primary olfactory center of the cockroach Periplaneta americana
Complete mapping of glomeruli based on sensory nerve branching pattern in the primary olfactory center of the cockroach Periplaneta americana
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DOI：
10.1002/cne.22452
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发表时间：
2010-10
影响因子：
2.5
通讯作者：
Hidehiro Watanabe;H. Nishino;M. Nishikawa;M. Mizunami;F. Yokohari
中科院分区：
文献类型：
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作者：
Hidehiro Watanabe;H. Nishino;M. Nishikawa;M. Mizunami;F. Yokohari
肾小球是脊椎动物和昆虫初级嗅觉中枢的结构和功能单位。美洲大蠊触角上的感受器中含有不同类型的感觉神经元，它们的轴突形成触角的背侧和腹侧神经，然后投射到许多肾小球。在这项研究中，我们确定了所有触角叶（AL）肾小球的基础上详细的神经支配模式的感觉束，除了形状，大小和位置的蟑螂。肾小球数量为10205，未观察到性别特异性差异。顺行染料注射到触角神经显示，供应AL的轴突分为10个感觉束（T1-T10）。每个T1-T3神经支配的小，椭圆形肾小球在前腹区的AL，从多个方向侵入每个肾小球的感觉传入，而每个T4-T10神经支配的大肾小球与各种形状的后背区，从一个方向侵入每个肾小球的感觉传入束。所有这些域的地形分支模式是保存在个人之间。在T10的亚束中的感觉传入在AL和前脑的背侧缘中具有轴突终末，在那里它们形成许多小的肾小球结构。感觉神经的分支模式反映了肾小球的发育过程，决定了肾小球的空间排列，因此基于感觉神经分支模式的完整的肾小球图谱将为研究肾小球空间排列的功能意义及其发育基础提供基础。J. Comp.神经元518：3907-3930，2010.© 2010 Wiley利斯公司
Glomeruli are structural and functional units in the primary olfactory center in vertebrates and insects. In the cockroach Periplaneta americana, axons of different types of sensory neurons housed in sensilla on antennae form dorsal and ventral antennal nerves and then project to a number of glomeruli. In this study, we identified all antennal lobe (AL) glomeruli based on detailed innervation patterns of sensory tracts in addition to the shape, size, and locations in the cockroach. The number of glomeruli is ∼205, and no sex‐specific difference is observed. Anterograde dye injections into the antennal nerves revealed that axons supplying the AL are divided into 10 sensory tracts (T1–T10). Each of T1–T3 innervates small, oval glomeruli in the anteroventral region of the AL, with sensory afferents invading each glomerulus from multiple directions, whereas each of T4–T10 innervates large glomeruli with various shapes in the posterodorsal region, with a bundle of sensory afferents invading each glomerulus from one direction. The topographic branching patterns of all these tracts are conserved among individuals. Sensory afferents in a sub‐tract of T10 had axon terminals in the dorsal margin of the AL and the protocerebrum, where they form numerous small glomerular structures. Sensory nerve branching pattern should reflect developmental processes to determine spatial arrangement of glomeruli, and thus the complete map of glomeruli based on sensory nerve branching pattern should provide a basis for studying the functional significance of spatial arrangement of glomeruli and its developmental basis. J. Comp. Neurol. 518:3907–3930, 2010. © 2010 Wiley‐Liss, Inc.