Mitigation of lead toxicity in Vigna radiata genotypes by silver nanoparticles
Mitigation of lead toxicity in Vigna radiata genotypes by silver nanoparticles
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DOI：
10.1016/j.envpol.2022.119606
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发表时间：
2022-09-01
影响因子：
8.9
通讯作者：
Lam, Su Shiung
中科院分区：
文献类型：
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作者：
Chen, Fu;Aqeel, Muhammad;Lam, Su Shiung
人类活动对土壤的重金属污染影响着生物系统和食物链。本研究旨在探讨银纳米颗粒（AgNPs）在两种基因型绿豆（Vigna radiata）（G1和G2）中的应用以改善铅的毒性。不同剂量的铅（0，25，50亩M）的差异处理银纳米粒子的目的是改善植物属性。这两种基因型表现出统计上显着的定量和定性调制铅耐受性。在G2阶段，与对照相比，L2（AgNPs 10 mg/L）处理的株高、鲜生物量和叶绿素总量的增加最为显著，分别为43.79%、49.56%和20%。总的来说，光合速率增加了26%，在G2在L 6（银纳米颗粒25毫克/L +铅25 μ M）。此外，结果表明，G2的水分利用效率增加了78.5%，而G1经历了最大的内部CO2浓度（209.8%）在L 8（铅50亩M）。AgNPs触发了对矿物质的平衡吸收，并改善了豇豆基因型的生长。50 μ M的Pb对豇豆的危害最大，沿着生长受到最大程度的抑制，光合活性受到显著抑制，G1期MDA含量增加199.7%，G2期H2 O2含量增加292.8%。与对照相比，最大的超氧化物歧化酶（376%），过氧化物酶（659.8%）和过氧化氢酶（9.3%）的活性在G2中观察到在L11。银纳米粒子的应用大大提高了植物的生长，并帮助他们在生存以及存在的情况下，铅。G2基因型表现出相当大的耐受能力，并揭示了所研究的属性比G1和银纳米颗粒的处理，即25 mg/L的最佳水平，在两种基因型中产生最好的结果较少的损害。结果表明，银纳米颗粒介导的响应（S）的植物在铅胁迫下，特别是有助于HM耐受性的植物，从而显示出巨大的希望，用于植物修复。
Heavy metal (HM) contamination of the soil through anthropogenic activities influences the living systems and drastically impacts food chain. This study examined the application of silver nanoparticles (AgNPs) in two genotypes (G1 and G2) of Mung bean (Vigna radiata) for ameliorating the Pb toxicity. Different doses of Pb (0, 25, 50 mu M) were differentially tackled by AgNPs with the aim of ameliorating the plant attributes. Both genotypes displayed statistically significant quantitative and qualitative modulations for Pb tolerance. In G2, the most prominent increase in plant height (43.79%), fresh biomass (49.56%) and total chlorophyll (20%) was observed at L2 (AgNPs 10 mg/L) in comparison with the control. Overall, photosynthetic rate was increased by 26% in G2 at L6 (AgNPs 25 mg/L + Pb 25 mu M). In addition, the results presented 78.5% increase in water use efficiency of G2 while G1 experienced a maximum internal CO2 concentration (209.8%) at L8 (Pb 50 mu M). AgNPs triggered balanced uptake of minerals and improved growth of Vigna genotypes. 50 mu M Pb was most hazardous and caused maximum reduction in growth of Vigna plants along with a significant suppression in photosynthetic activity, increase in MDA (199.7%) in G1 and H2O2 (292.8%) in G2. In comparison to control, maximum superoxide dismutase (376%), peroxidase (659.8%) and catalase (9.3%) activity was observed in G2 at L11. The application of AgNPs substantially enhanced plant growth and helped them in surviving well in absence as well as presence of Pb. G2 genotype exhibited substantial tolerance capability and revealed less impairment in the studied attributes than G1 and treatment of AgNPs i.e. 25 mg/L was the best level that yielded best results in both genotypes. The results demonstrate that AgNPs mediate response(s) of plants under Pb stress and particularly contributed to HM tolerance of plants and thus showing great promise for use in phytoremediation.