The increased expression of fatty acid-binding protein 9 in prostate cancer and its prognostic significance.
The increased expression of fatty acid-binding protein 9 in prostate cancer and its prognostic significance.
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DOI：
10.18632/oncotarget.12635
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发表时间：
2016-12-13
期刊：
影响因子：
--
通讯作者：
Ke Y
中科院分区：
文献类型：
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作者：
Al Fayi MS;Gou X;Forootan SS;Al-Jameel W;Bao Z;Rudland PR;Cornford PA;Hussain SA;Ke Y
与研究脂肪酸结合蛋白5（FABP 5）在前列腺癌中的关键作用的许多研究相反，对其他FABPs可能参与的研究很少。在此，我们首先测量了10种FABPs在良性和恶性前列腺细胞系中的mRNA水平，并鉴定了差异表达的FABP 6和FABP 9 mRNA，其在所有恶性细胞系中的水平均高于良性细胞中的水平。此后，我们评估了FABP 6和FABP 9在两种前列腺细胞系和人体组织中的表达状态。FABP 6蛋白仅在5种恶性细胞系中的1种中过表达，其免疫染色强度在良性和恶性前列腺组织中无明显差异。相反，FABP 9蛋白在高度恶性细胞系PC-3和PC 3-M中高表达，但在良性PNT-2和其他恶性细胞系中未检测到其水平。当在人类前列腺组织的档案集中进行分析时，癌中FABP 9的免疫组织化学染色强度显著高于良性病例，并且FABP 9的增加与患者生存时间的减少显著相关。此外，FABP 9染色水平的增加与关节Gleason评分（GS）和雄激素受体指数（AR）的增加显著相关。在高度恶性的PC 3-M细胞中抑制FABP 9表达可抑制其侵袭潜力。我们的研究结果表明，FABP 9是一个有价值的预后标志物，预测前列腺癌患者的结果，可能在前列腺癌细胞侵袭中发挥重要作用。
In contrast to numerous studies conducted to investigate the crucial role of fatty acid binding protein 5 (FABP5) in prostate cancer, investigations on the possible involvement of other FABPs are rare. Here we first measured the mRNA levels of 10 FABPs in benign and malignant prostate cell lines and identified the differentially expressed FABP6 and FABP9 mRNAs whose levels in all malignant cell lines were higher than those in the benign cells. Thereafter we assessed the expression status of FABP6 and FABP9 in both prostate cell lines and in human tissues. FABP6 protein was overexpressed only in 1 of the 5 malignant cell lines and its immunostaining intensities were not significantly different between benign and malignant prostate tissues. In contrast, FABP9 protein was highly expressed in highly malignant cell lines PC-3 and PC3-M, but its level in the benign PNT-2 and other malignant cell lines was not detectable. When analysed in an archival set of human prostate tissues, immunohistochemical staining intensity for FABP9 was significantly higher in carcinomas than in benign cases and the increase in FABP9 was significantly correlated with reduced patient survival times. Moreover, the increased level of staining for FABP9 was significantly associated with the increased joint Gleason scores (GS) and androgen receptor index (AR). Suppression of FABP9 expression in highly malignant PC3-M cells inhibited their invasive potential. Our results suggest that FABP9 is a valuable prognostic marker to predict the outcomes of prostate cancer patients, perhaps by playing an important role in prostate cancer cell invasion.