ICE1:: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis
ICE1:: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis
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DOI：
10.1101/gad.1077503
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发表时间：
2003-04-15
影响因子：
10.5
通讯作者：
Zhu, JK
中科院分区：
文献类型：
--
作者：
Chinnusamy, V;Ohta, M;Zhu, JK
低温触发CBF家族转录因子的表达，这反过来又激活了许多下游基因，这些基因赋予植物耐冷性和抗冻性。我们在这里报告的识别ICE 1（诱导CBF表达1），上游转录因子，调节CBF基因在寒冷的转录。在筛选损害CBF3启动子荧光素酶报告基因冷诱导转录的突变中分离出拟南芥ice 1突变体。ice1突变阻断了CBF3的表达，降低了CBF下游许多基因的表达，导致植物耐冷性和抗冻性显著降低。ICE1编码MYC样bHLH转录激活因子。ICE1特异性结合CBF3启动子中的MYC识别序列。ICE 1是组成型表达的，其在野生型植物中的过表达增强了CBF调节子在寒冷中的表达，并提高了转基因植物的抗冻性。
Cold temperatures trigger the expression of the CBF family of transcription factors, which in turn activate many downstream genes that confer chilling and freezing tolerance to plants. We report here the identification of ICE1 (inducer of CBF expression 1), an upstream transcription factor that regulates the transcription of CBF genes in the cold. An Arabidopsis ice1 mutant was isolated in a screen for mutations that impair cold-induced transcription of a CBF3 promoter-luciferase reporter gene. The ice1 mutation blocks the expression of CBF3 and decreases the expression of many genes downstream of CBFs, which leads to a significant reduction in plant chilling and freezing tolerance. ICE1 encodes a MYC-like bHLH transcriptional activator. ICE1 binds specifically to the MYC recognition sequences in the CBF3 promoter. ICE1 is expressed constitutively, and its overexpression in wild-type plants enhances the expression of the CBF regulon in the cold and improves freezing tolerance of the transgenic plants.