Structural insights into the elevator-type transport mechanism of a bacterial ZIP metal transporter.
Structural insights into the elevator-type transport mechanism of a bacterial ZIP metal transporter.
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DOI：
10.1038/s41467-023-36048-4
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发表时间：
2023-01-24
影响因子：
16.6
通讯作者：
Hu, Jian
中科院分区：
文献类型：
--
作者：
Zhang, Yao;Jiang, Yuhan;Gao, Kaifu;Sui, Dexin;Yu, Peixuan;Su, Min;Wei, Guo-Wei;Hu, Jian
Zrt-/ irt样蛋白（ZIP）家族由普遍表达的二价金属转运蛋白组成，在维持锌、铁和锰的系统和细胞稳态中起关键作用。本文采用结构生物学、进化协方差、计算建模和多种生化分析相结合的方法，对一株原核细菌细菌（Bordetella bronchiseptica, BbZIP）进行了研究，以解决ZIP家族尚未确定的转运机制问题。向内构象的载脂蛋白状态结构揭示了一个拆卸的运输位点，改变了螺旋间的相互作用，重要的是，相对于其他TM， 4-跨膜螺旋束（TM）的刚体运动。计算生成和生化验证的面向外的构象模型显示，携带转运位点的4-TM束向细胞外侧滑动约8 Å，反对介导二聚化的静态tm。这些发现使我们得出结论，BbZIP是一种电梯式运输机。ZIP家族（SLC39A）参与过渡金属稳态。在这里，作者使用综合方法得出结论，支气管杆菌的ZIP （BbZIP）利用电梯式运输机制实现交替进入。
The Zrt-/Irt-like protein (ZIP) family consists of ubiquitously expressed divalent metal transporters critically involved in maintaining systemic and cellular homeostasis of zinc, iron, and manganese. Here, we present a study on a prokaryotic ZIP from Bordetella bronchiseptica (BbZIP) by combining structural biology, evolutionary covariance, computational modeling, and a variety of biochemical assays to tackle the issue of the transport mechanism which has not been established for the ZIP family. The apo state structure in an inward-facing conformation revealed a disassembled transport site, altered inter-helical interactions, and importantly, a rigid body movement of a 4-transmembrane helix (TM) bundle relative to the other TMs. The computationally generated and biochemically validated outward-facing conformation model revealed a slide of the 4-TM bundle, which carries the transport site(s), by approximately 8 Å toward the extracellular side against the static TMs which mediate dimerization. These findings allow us to conclude that BbZIP is an elevator-type transporter. The ZIP family (SLC39A) is involved in transition metal homeostasis. Here, authors use integrated approaches to conclude that the ZIP from Bordetella bronchiseptica (BbZIP) utilizes the elevator-type transport mechanism to achieve alternating access.
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DOI：
10.1073/pnas.1222142110
发表时间：
2013-06-11
影响因子：
11.1
作者：
Chowanadisai, Winyoo;Graham, David M.;Messerli, Mark A.
通讯作者：
Messerli, Mark A.
DOI：
10.1073/pnas.0804659105
发表时间：
2008-07-29
影响因子：
11.1
作者：
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通讯作者：
Rudnick, Gary
影响因子：
5.7
作者：
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通讯作者：
Dalbey, Ross E.
影响因子：
6.7
作者：
Crawford AC;Lehtovirta-Morley LE;Alamir O;Niemiec MJ;Alawfi B;Alsarraf M;Skrahina V;Costa ACBP;Anderson A;Yellagunda S;Ballou ER;Hube B;Urban CF;Wilson D
通讯作者：
Wilson D
影响因子：
2.9
作者：
Antala, Sagar;Dempski, Robert E.
通讯作者：
Dempski, Robert E.