Visualisation of 'Marx's line' along the marginal eyelid conjunctiva of human subjects with lissamine green dye
Visualisation of 'Marx's line' along the marginal eyelid conjunctiva of human subjects with lissamine green dye
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DOI：
10.1046/j.1475-1313.2003.00160.x
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发表时间：
2004-01-01
影响因子：
2.9
通讯作者：
Button, NF
中科院分区：
文献类型：
--
作者：
Doughty, MJ;Naase, T;Button, NF
目的：定性评估丽丝胺绿染料对马克思线的染色频率和强度。方法：对 85 名年龄在 18-78 岁、白人（欧洲）或阿拉伯裔的非接触镜佩戴者进行了评估。受试者没有严重的眼部疾病，尽管有些受试者有轻度至中度的主要睑板腺充血和/或前睑缘炎。使用丽丝胺绿染料条对睑结膜边缘区进行染色，然后拍摄高倍裂隙灯照片。结果：83 名受试者的丽丝胺绿染色线是明确无误的，而其他两名受试者的丽丝胺绿染色线则很明显。该线沿着上下眼睑边缘的整个长度延伸，并且染色变宽并延伸到泪点。在大多数受试者中，丽丝胺绿染色线位于主要睑板腺孔的后面，但在五个受试者中，它明显位于跗骨孔的前面。在超过三分之二的受试者中，线的宽度远小于腺体孔之间的距离，并且与腺孔之间的距离相似。然而，在三分之一的受试者中，线的宽度约为孔口之间距离的一半或稍大（即约 0.5 毫米），并且此特征在非欧洲血统的受试者中更常见。结论：丽丝胺绿染料突出显示了沿边缘区域的非常明显的细胞线，并且应该是可视化此特征的有用工具。该细胞系被认为是眼睑边缘与球结膜和角膜表面之间的自然接触部位。
Purpose: To qualitatively assess the frequency and intensity of the staining of Marx's line with lissamine green dye.Methods: Eighty-five non-contact lens wearers, aged 18-78 years and of white (European) or Arabic origin, were assessed. The subjects had no major eye disease, although some had mild-to-moderate congestion of the main tarsal (Meibomian) glands and/or anterior blepharitis. Lissamine green dye strips were used to stain the marginal zone of the palpebral conjunctiva, and then high magnification photo slitlamp images were taken.Results: A lissamine green staining line was unmistakable in 83 of the subjects, and just evident in the other two. The line extends along the entire length of the upper and lower lid margins, and the staining broadens and extends over the puncta. In most subjects, the lissamine green-stained line was posterior to the main tarsal gland orifices, but in five subjects it was distinctly anterior to the orifices. In over two-thirds of the subjects, the width of line was much less than the distance between the gland orifices, and was at a similar distance to its width from the orifices. However, in one-third of the subjects, the width of the line was around half that of the distance between the orifices or slightly greater (i.e. approximately 0.5 mm), and this feature was more commonly present in those of non-European origin.Conclusion: Lissamine green dye highlights a very distinct line of cells along the marginal zone, and should be a useful tool to visualise this feature. This line of cells is thought to be the natural site of contact between the eyelid margin and the surfaces of the bulbar conjunctiva and cornea.