The interaction of Sendai virus with negatively charged liposomes: virus-induced lysis of carboxyfluorescein-loaded small unilamellar vesicles.
The interaction of Sendai virus with negatively charged liposomes: virus-induced lysis of carboxyfluorescein-loaded small unilamellar vesicles.
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仙台病毒与带负电荷的脂质体的相互作用：病毒诱导的负载羧基荧光素的小单层囊泡的裂解。
DOI：
10.1016/0005-2736(85)90208-1
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Barenholz,Y
中科院分区：
文献类型：
--
作者：
Amselem,S;Loyter,A;Lichtenberg,D;Barenholz,Y
研究了仙台病毒与小的单层囊泡的相互作用，所述囊泡缺乏病毒受体并且装载有自猝灭的6-羧基荧光素。仙台病毒体诱导释放的羧基荧光素囊泡组成的带负电荷的磷脂，尽管事实上，他们不包含病毒受体。初步实验表明，羧基荧光素的释放伴随着病毒和脂质体脂质及其截留内容物的混合，这表明脂质体-病毒融合。没有释放的羧基荧光素，观察囊泡只含有磷脂酰胆碱。病毒诱导的羧基荧光素释放速率是温度依赖性的;病毒的裂解活性在25°C以上大大增强。这种效应不是由于脂质囊泡或病毒体中的脂质的热相变。病毒诱导的羧基荧光素释放被抑制的存在下，在介质中的钙离子和胆固醇的脂质囊泡。随着脂质囊泡或病毒体浓度的增加，它也增加。用增加浓度的三种不同的蛋白水解酶（胰蛋白酶、胰凝乳蛋白酶和蛋白酶）预处理病毒粒子抑制病毒引起从带负电荷的脂质体释放羧基荧光素的能力。在高于56°C孵育病毒体后，也观察到病毒裂解活性的抑制。
The interaction of Sendai virus with small, unilamellar vesicles, lacking virus receptors and loaded with self-quenched 6-carboxyfluorescein, was studied. Sendai virions induced release of carboxyfluorescein from vesicles composed of negative charged phospholipids, despite the fact that they did not contain virus receptors. Preliminary experiments indicate that the carboxyfluorescein release is accompanied by mixing of the virus and liposome lipids and their entrapped contents, suggesting liposome-virus fusion. No release of carboxyfluorescein was observed with vesicles containing only phosphatidylcholine. The rate of virus-induced carboxyfluorescein release was temperature dependent; the lytic activity of the virus was greatly enhanced above 25°C. This effect was not due to a thermal phase transition of the lipids in either the lipid vesicles or the virions. Virus-induced carboxyfluorescein release was inhibited by the presence of calcium ions in the medium and of cholesterol in the lipid vesicles. It increased with increasing concentrations of either the lipid vesicles or the virions. Pretreatment of virions with increasing concentrations of three different proteolytic enzymes (trypsin, chymotrypsin and proteinase) inhibited the virus' ability to cause release of carboxyfluorescein from negatively charged liposomes. Inhibition of the viral lytic activity was also observed after virions were incubated above 56°C.