Three-stage hydrolysis to enhance enzymatic saccharification of steam-exploded corn stover
Three-stage hydrolysis to enhance enzymatic saccharification of steam-exploded corn stover
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三级水解增强汽爆玉米秸秆酶促糖化
DOI：
10.1016/j.biortech.2009.09.079
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发表时间：
2010-07-01
影响因子：
11.4
通讯作者：
Yu, Shiyuan
中科院分区：
文献类型：
--
作者：
Yang, Jing;Zhang, Xiaoping;Yu, Shiyuan
本研究旨在探索缩短酶促糖化反应时间、提高酶促糖化效率的新途径。蒸汽爆裂玉米秸秆的一段水解期为72 h，得率为62.8%，而多级水解法可将水解率降至24 h，因此提出了三段水解法的概念，即纤维素底物分别水解6、6、12 h。在24 h内获得了较高的水解率，酶循环使用时的水解率为70.2%，添加新鲜酶消除酶回收时的水解率为76.1%，分析表明，短时间的水解液和各阶段最终产物的去除提高了纤维素酶的活力，有利于纤维素酶在固体底物上的吸附。当用蒸汽爆裂的玉米秸秆作为纤维素酶合成的底物时，24小时内水解率达到88.6%。
The objective of the present research was to explore new approach to reduce the hydrolysis time and to enhance the productivity of enzymatic saccharification. One-stage hydrolysis of steam-exploded corn stover required 72 h to reach a yield of 62.8%, while multi-stage hydrolysis could reduce the time to 24 h. A concept of three-stage hydrolysis was therefore proposed in which cellulosic substrate was hydrolyzed for 6, 6, and 12 h, respectively. High hydrolysis yields, 70.2% with enzyme recycling and 76.1% with the supplement of fresh enzyme to eliminate enzyme recovery procedure, were obtained in 24 h. Analysis indicated that short-time hydrolysis and the removal of end products at each stage improved cellulase activities and benefited the adsorption of cellulase enzyme to the solid substrate. When steam-exploded corn stover was used as the substrate for cellulase synthesis, a hydrolysis yield of 88.6% was achieved in 24 h. (C) 2009 Elsevier Ltd. All rights reserved.