Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry
Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry
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DOI：
10.1016/j.diagmicrobio.2012.12.009
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发表时间：
2013-04-01
影响因子：
2.9
通讯作者：
Rentenaar, Rob J.
中科院分区：
文献类型：
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作者：
de Jong, Eefje;de Jong, Arjan S.;Rentenaar, Rob J.
采用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF MS)对99株临床分离的催产克雷伯菌进行了鉴定。经鉴定有8个菌株为Raoulella spp.，其中5个为Raoulella spp.经16S rRNA测序，得到3株催产克雷伯菌。通过对MALDI-TOF MS分值应用10%的差异规则，这些分离物得到了正确的鉴定。这种方法可能有助于区分拉氏菌和催产克雷氏菌。(C)2013 Elsevier Inc.保留所有权利。
Ninety-nine clinical isolates previously identified as Klebsiella oxytoca were evaluated using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight isolates were identified as Raoultella spp., being 5 Raoultella spp. and 3 K. oxytoca, by 16S rRNA sequencing. These isolates were correctly identified by applying the 10% differential rule for the MALDI-TOF MS score values. This approach might be useful to discriminate Raoultella species from K. oxytoca. (C) 2013 Elsevier Inc. All rights reserved.