High-resolution macromolecular structure determination using CCD detectors and synchrotron radiation.
High-resolution macromolecular structure determination using CCD detectors and synchrotron radiation.
复制标题
使用 CCD 探测器和同步加速器辐射进行高分辨率大分子结构测定。
DOI：
10.1016/s0969-2126(01)00218-0
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ealick,SE
中科院分区：
文献类型：
--
作者：
Walter,RL;Thiel,DJ;Barna,SL;Tate,MW;Wall,ME;Eikenberry,EF;Gruner,SM;Ealick,SE
背景：同步辐射光源对大分子晶体学做出了令人印象深刻的贡献。然而，在开发适当的X射线探测器方面的延迟严重限制了它们的有效使用。新技术，电荷耦合器件（CCD）的基础上，提供更快，更准确，自动化datacollection.ResultsA CCD为基础的X射线探测器的能力已经开发用于在大分子晶体学，并已在约一年半的康奈尔高能量同步辐射源的操作。它已被用于各种晶体学项目，包括一些高分辨率的结构研究。数据的统计质量，检测器的易用性和使用效率，以及越来越多的结构结果说明了这种新的检测器system.ConclusionThe新的检测器的实际效用有增强的能力，用于测量衍射图案从晶体的大分子，特别是在高分辨率，当X射线强度弱。这里描述的调查结果的范围从病毒晶体学弱衍射小分子结构的测定，并展示了CCD探测器的潜力时，结合同步辐射源。
Background:Synchrotron radiation sources have made impressive contributions to macromolecular crystallography. The delay in development of appropriate X-ray detectors has, however, been a significant limitation to their efficient use. New technologies, based on charge-coupled devices (CCDs), provide capabilities for faster, more accurate, automated data collection.ResultsA CCD-based X-ray detector has been developed for use in macromolecular crystallography and has been in operation for about one and a half years at the Cornell High Energy Synchrotron Source. It has been used for a variety of crystallographic projects, including a number of high-resolution structural studies. The statistical quality of the data, the detector's ease and efficiency of use, and the growing number of structural results illustrate the practical utility of this new detector system.ConclusionThe new detector has enhanced capabilities for measuring diffraction patterns from crystals of macromolecules, especially at high resolution, when the X-ray intensities are weak. The survey of results described here ranges from virus crystallography to weakly diffracting small-molecule structure determination and demonstrates the potential of CCD detectors when combined with synchrotron radiation sources.