Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo
Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo
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DOI：
10.1016/j.molcel.2019.04.014
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发表时间：
2019-07-11
期刊：
影响因子：
16
通讯作者：
Corces, Victor G.
中科院分区：
文献类型：
--
作者：
Jung, Yoon Hee;Kremsky, Isaac;Corces, Victor G.
哺乳动物配子中存在的表观遗传信息以及它是否传递给后代是相对未知的。我们发现小鼠精子中的许多启动子被RNA聚合酶II(Pol II)和介体占据。在GV和MII卵母细胞和植入前胚胎中也可以获得相同的启动子。包含各种转录因子基序的精子远端ATAC-SEQ位点在猴子和人类中是保守的。CHIP-SEQ分析证实FOXA1、ERα和AR在精子中占据远端增强子。含有H3.3和H2A.Z的可及性精子增强剂也可在卵母细胞和植入前胚胎中获得。此外，它们与配子中的启动子的相互作用在早期发育过程中持续存在。由CTCF和粘附素介导的精子或卵母细胞特异性相互作用仅存在于受精卵和2-细胞期的父本或母本染色体中。这些相互作用在8-细胞期时在两条染色体上汇聚。因此，哺乳动物配子包含复杂的3D相互作用模式，这些模式可以在受精后传递给受精卵。
The epigenetic information present in mammalian gametes and whether it is transmitted to the progeny are relatively unknown. We find that many promoters in mouse sperm are occupied by RNA polymerase II (Pol II) and Mediator. The same promoters are accessible in GV and MII oocytes and preimplantation embryos. Sperm distal ATAC-seq sites containing motifs for various transcription factors are conserved in monkeys and humans. ChIP-seq analyses confirm that Foxa1, ER alpha, and AR occupy distal enhancers in sperm. Accessible sperm enhancers containing H3.3 and H2A.Z are also accessible in oocytes and preimplantation embryos. Furthermore, their interactions with promoters in the gametes persist during early development. Sperm-or oocyte-specific interactions mediated by CTCF and cohesin are only present in the paternal or maternal chromosomes, respectively, in the zygote and 2-cell stages. These interactions converge in both chromosomes by the 8-cell stage. Thus, mammalian gametes contain complex patterns of 3D interactions that can be transmitted to the zygote after fertilization.