An Ultrahigh Resolution SPECT System for I-125 Mouse Brain Imaging Studies.
An Ultrahigh Resolution SPECT System for I-125 Mouse Brain Imaging Studies.
复制标题
用于 I-125 小鼠脑成像研究的超高分辨率 SPECT 系统。
DOI：
10.1016/j.nima.2008.11.149
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Chen,CT
中科院分区：
文献类型：
--
作者：
Meng,LJ;Fu,G;Roy,EJ;Suppe,B;Chen,CT
本文介绍了通过双头原型单光子发射显微镜系统 (SPEM) 获得的一些初步实验结果，该系统专用于使用 I-125 标记的放射性示踪剂进行小鼠大脑研究。特别是，该系统将用于体内定位小鼠大脑中放射性标记的 T 细胞。该系统基于增强电子倍增电荷耦合器件（I-EMCCD）相机的使用，该相机在27-140keV的能量范围内提供了出色的固有空间分辨率、良好的信噪比、大的活动面积和合理的检测效率。在本研究中，使用分辨率模型和局部注射 I-125 标记 T 细胞的小鼠对双头 SPEM 系统进行了评估。结果表明，对于相对集中的源物体，当前的双头 SPEM 系统能够可视化极少数（<1000）T 细胞携带的微量放射性（∼12nCi）。当前的 SPEM 系统设计允许在固定系统配置中安装四个或六个摄像头，从而以与双头系统类似的空间分辨率提供双倍或三倍的灵敏度。这一进展将为小鼠大脑中放射性标记 T 细胞的体内和非侵入性追踪提供强大的工具，并可能为其他啮齿动物脑成像研究提供强大的工具。
This paper presents some initial experimental results obtained with a dual-head prototype single photon emission microscope system (SPEM) that is dedicated to mouse brain studies using I-125 labeled radiotracers. In particular, this system will be used for in vivo tacking of radiolabeled T cells in mouse brain. This system is based on the use of the intensified electron multiplying charge-coupled device (I-EMCCD) camera that offers the combination of an excellent intrinsic spatial resolution, a good signal-to-noise ratio, a large active area and a reasonable detection efficiency over an energy range between 27–140keV. In this study, the dual-head SPEM system was evaluated using both resolution phantoms and a mouse with locally injected T cells labeled with I-125. It was demonstrated that for a relatively concentrated source object, the current dual-head SPEM system is capable of visualizing the tiny amount of radioactivity (∼12nCi) carried by a very small number (<1000) of T cells. The current SPEM system design allows four or six camera heads to be installed in a stationary system configuration that offers a doubled or tripled sensitivity at a spatial resolution similar to that obtained with the dual-head system. This development would provide a powerful tool for in vivo and non-invasive tracking of radiolabeled T cells in mouse brain and potentially for other rodent brain imaging studies.