Effect of topography of an electrospun nanofiber on modulation of activity of primary rat astrocytes
Effect of topography of an electrospun nanofiber on modulation of activity of primary rat astrocytes
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DOI：
10.1016/j.neulet.2012.11.015
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发表时间：
2013-02-08
影响因子：
2.5
通讯作者：
Shin, Hwa Sung
中科院分区：
文献类型：
--
作者：
Min, Seul Ki;Kim, Sung Hoon;Shin, Hwa Sung
最近提出了几种用于神经组织工程的生物材料，用于受损组织的再生和中枢神经系统损伤后促进轴突引导。当植入受损的神经组织时，生物材料应有利于诱导细胞浸润和轴突引导，同时抑制炎症。纳米纤维支架被认为是满足上述要求的充分材料；然而，迄今为止对这些材料进行的大多数研究都针对神经元细胞，而不是神经胶质细胞，尽管它们在受损的中枢神经系统中具有重要功能。在这项研究中，研究了聚己内酯（PCL）的电纺纳米纤维支架的形貌对星形胶质细胞行为和 GFAP 表达的影响。结果表明，PCL纳米纤维拓扑促进粘附，但GFAP表达下调，导致星形胶质细胞活性降低。综上所述，这些结果表明，电纺纳米纤维的形貌结构提供了一个支架，通过减轻星形胶质细胞增生有利于神经再生。 (C) 2012 Elsevier Ireland Ltd. 版权所有。预订的。
Several biomaterials for neural tissue engineering have recently been proposed for regeneration of damaged tissue and promotion of axonal guidance following CNS injury. When implanted into damaged nerve tissue, biomaterials should favorably induce cell infiltration and axonal guiding while suppressing inflammation. Nanofiber scaffolds are regarded as adequate materials to meet the above requirements; however, most studies of these materials conducted to date have targeted neuronal cells, not glial cells, despite their important function in the injured CNS. In this study, an electrospun nanofibrous scaffold of polycaprolactone (PCL) was investigated with respect to its topographic effects on astrocyte behavior and expression of GFAP. The results revealed that the PCL nanofiber topograghy promoted adhesion, but GFAP expression was down-regulated, leading to reduced astrocytes activity. Taken together, these results indicate that the topographic structure of electrospun nanofibers provides a scaffold that is favorable to neural regeneration via alleviation of astrogliosis. (C) 2012 Elsevier Ireland Ltd. All rights. reserved.