Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae.
Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae.
复制标题
人乳头瘤病毒 58 型基因组在酿酒酵母中的复制和转录
DOI：
10.1186/1743-422x-7-368
复制
发表时间：
2010-12-15
期刊：
影响因子：
4.8
通讯作者：
Zhao WM
中科院分区：
文献类型：
--
作者：
Li J;Wang X;Liu J;Wang H;Zhang XL;Tang W;Sun YD;Wang X;Yu XP;Zhao WM
背景
为了建立一个方便的研究人乳头瘤病毒（HPV）的系统，我们将酿酒酵母选择标记Ura插入HPV 58基因组中，并将其转化到酵母中。
结果
HPV58基因组可在酵母细胞中进行染色体外复制，其早期和晚期基因均有转录。然而，随着病毒E2基因的突变，HPV58基因组失去了有丝分裂的稳定性，E6和E7基因的转录水平上调。
结论
E2蛋白参与病毒基因组的维持、复制和转录调控。该酵母模型可用于研究HPV生命周期的某些方面。
Background
To establish a convenient system for the study of human papillomavirus (HPV), we inserted a Saccharomyces cerevisiae selectable marker, Ura, into HPV58 genome and transformed it into yeast.
Results
HPV58 genome could replicate extrachromosomally in yeast, with transcription of its early and late genes. However, with mutation of the viral E2 gene, HPV58 genome lost its mitotic stability, and the transcription levels of E6 and E7 genes were upregulated.
Conclusions
E2 protein could participate in viral genome maintenance, replication and transcription regulation. This yeast model could be used for the study of certain aspects of HPV life cycle.