Quantitative analysis of the spatiotemporal dynamics of a synthetic predator-prey ecosystem.
Quantitative analysis of the spatiotemporal dynamics of a synthetic predator-prey ecosystem.
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DOI：
10.1007/978-1-61779-412-4_19
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发表时间：
2012
影响因子：
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通讯作者：
Stephen Payne;R. P. Smith;L. You
中科院分区：
文献类型：
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作者：
Stephen Payne;R. P. Smith;L. You
合成生物学的一个主要关注点是合理设计和实现基因电路，以控制单个细胞和越来越多的细胞群体的动态。在试图设计和实施合成生态系统(或工程微生物联合体)的最近研究中，强调了种群水平的控制。一方面，这些工程系统可能成为实际应用的关键技术基础。另一方面，它们可以作为明确定义的模型系统来研究具有广泛相关性的生物学问题。在这里，我们以一个合成的捕食者-被捕食者生态系统为例，说明了系统实现和表征所涉及的基本实验技术。因此，这些技术也适用于其他以微生物为基础的合成或自然生态系统的分析。
A major focus in synthetic biology is the rational design and implementation of gene circuits to control dynamics of individual cells and, increasingly, cellular populations. Population-level control is highlighted in recent studies which attempt to design and implement synthetic ecosystems (or engineered microbial consortia). On the one hand, these engineered systems may serve as a critical technological foundation for practical applications. On the other hand, they may serve as well-defined model systems to examine biological questions of broad relevance. Here, using a synthetic predator–prey ecosystem as an example, we illustrate the basic experimental techniques involved in system implementation and characterization. By extension, these techniques are applicable to the analysis of other microbial-based synthetic or natural ecosystems.