Construction of an in vivo system for functional analysis of the genes involved in sex pheromone production in the silkmoth, Bombyx mori.
Construction of an in vivo system for functional analysis of the genes involved in sex pheromone production in the silkmoth, Bombyx mori.
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DOI：
10.3389/fendo.2012.00030
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发表时间：
2012
影响因子：
5.2
通讯作者：
Matsumoto S
中科院分区：
文献类型：
--
作者：
Moto K;Matsumoto S
蛾产生种特异性信息素来吸引同种的配偶。包括性信息素生物合成的生物化学过程被精确地调节，并且许多基因产物参与该生物合成和调节。近年来，从家蚕信息素腺体（PG）特异性cDNA文库中分离出至少300个EST克隆，其中一些克隆[即，B。桑PG特异性去饱和酶1（Bmpgdesat 1），PG特异性脂肪酰基还原酶，PG特异性酰基辅酶A结合蛋白，B.桑脂肪酸转运蛋白，B.桑脂储存液滴蛋白-1]的特征在于并证明在性信息素的产生中发挥作用。然而，大多数EST克隆尚未完全表征和鉴定。为了开发一个有效的系统分析性信息素生产相关的基因，我们研究了一种新的基因分析系统的可行性，使用的上游区域Bmpgdesat 1，应该包含PG特异性基因启动子结合piggyBac载体介导的生殖系转化。结果，我们已经能够获得我们的报告基因（增强型绿色荧光蛋白）在PG中的表达，而不是在转基因B的其他组织中。桑。目前的结果表明，我们已经成功构建了一种新型的B性信息素产生的体内基因分析系统，用于B。森。
Moths produce species-specific sex pheromones to attract conspecific mates. The biochemical processes that comprise sex pheromone biosynthesis are precisely regulated and a number of gene products are involved in this biosynthesis and regulation. In recent years, at least 300 EST clones have been isolated from Bombyx mori pheromone gland (PG) specific cDNA libraries with some of those clones [i.e., B. mori PG-specific desaturase 1 (Bmpgdesat1), PG-specific fatty acyl reductase, PG-specific acyl-CoA-binding protein, B. mori fatty acid transport protein, B. mori lipid storage droplet protein-1] characterized and demonstrated to play a role in sex pheromone production. However, most of the EST clones have yet to be fully characterized and identified. To develop an efficient system for analyzing sex pheromone production-related genes, we investigated the feasibility of a novel gene analysis system using the upstream region of Bmpgdesat1 that should contain a PG-specific gene promoter in conjunction with piggyBac vector-mediated germ line transformation. As a result, we have been able to obtain expression of our reporter gene (enhanced green fluorescent protein) in the PG but not in other tissues of transgenic B. mori. Current results indicate that we have successfully constructed a novel in vivo gene analysis system for sex pheromone production in B. mori.